Mon Aug 31 2015 13:52:38 GMT+0200 (CEST)2025-05-10 03:53:08
locus
BSU04970
BSU_04970
essential
ec
outlinks
bsu
BSU04970
BSU_04970
syns
yddH
[SW|Categories] containing this gene/protein
[SW|mobile genetic elements], [SW|cell wall degradation/ turnover]
The protein
Catalyzed reaction/ biological activity
hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D-γ-glutamate and meso-diaminopimelic acid) [Pubmed|18305117]
hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D--glutamate and meso-diaminopimelic acid) [Pubmed|18305117]
degradation of gamma-polyglutamic acid [pubmed|29458655]
The protein
Protein family
nlpC/p60 family (according to Swiss-Prot)
[SW|Peptidase C40 family] (according to UniProt)
The protein
[SW|Domains]
N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine [Pubmed|18305117]
C-terminal endopeptidase domain cleaves the bond between D-γ-glutamate and meso-diaminopimelic acid [Pubmed|18305117]
N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine [Pubmed|18305117]
C-terminal D,L-endopeptidase domain ([SW|NlpC/P60 domain]) [pubmed|29458655,22139507], cleaves the bond between D--glutamate and meso-diaminopimelic acid [Pubmed|18305117]
Expression and Regulation
Regulation
induced by mitomycin C [Pubmed|18305117]
References
proteinLength
329
geneLength
990
Gene
Coordinates
544,022 545,011
The protein
Paralogous protein(s)
[[this]]
The protein
Effectors of protein activity
both enzymatic activities are inhibited by interaction with [[protein|IseA]] [pubmed|29458655]
The protein
Structure
[PDB|4FDY] (from Staphylococcus aureus, 42% identity) [pubmed|24051416]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE04970 ([[gene|cwlT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA
BKK04970 ([[gene|cwlT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA