SubtiBank SubtiBank
Version comparison:

Mon Aug 31 2015 13:52:38 GMT+0200 (CEST)2025-05-10 03:53:08

locus

BSU04970

BSU_04970

essential

ec

outlinks

bsu

BSU04970

BSU_04970

syns

yddH

[SW|Categories] containing this gene/protein

[SW|mobile genetic elements], [SW|cell wall degradation/ turnover]

The protein

Catalyzed reaction/ biological activity

hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D-γ-glutamate and meso-diaminopimelic acid) [Pubmed|18305117]

hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D--glutamate and meso-diaminopimelic acid) [Pubmed|18305117]

degradation of gamma-polyglutamic acid [pubmed|29458655]

The protein

Protein family

nlpC/p60 family (according to Swiss-Prot)

[SW|Peptidase C40 family] (according to UniProt)

The protein

[SW|Domains]

N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine [Pubmed|18305117]

C-terminal endopeptidase domain cleaves the bond between D-γ-glutamate and meso-diaminopimelic acid [Pubmed|18305117]

N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine [Pubmed|18305117]

C-terminal D,L-endopeptidase domain ([SW|NlpC/P60 domain]) [pubmed|29458655,22139507], cleaves the bond between D--glutamate and meso-diaminopimelic acid [Pubmed|18305117]

Expression and Regulation

Regulation

induced by mitomycin C [Pubmed|18305117]

References

18305117, 24532767

18305117, 24532767, 24051416, 29458655, 32419322

proteinLength

329

geneLength

990

Gene

Coordinates

544,022 545,011

The protein

Paralogous protein(s)

[[this]]

The protein

Effectors of protein activity

both enzymatic activities are inhibited by interaction with [[protein|IseA]] [pubmed|29458655]

The protein

Structure

[PDB|4FDY] (from Staphylococcus aureus, 42% identity) [pubmed|24051416]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE04970 ([[gene|cwlT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA

BKK04970 ([[gene|cwlT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATCTTGTTCTTTCATC, downstream forward: _UP4_ATTAAATAACTAGGAGTGAA