Tue Dec 13 2016 11:16:33 GMT+0100 (CET)2025-05-10 22:29:53
locus
BSU15630
BSU_15630
ec
names
ylnF
ylnF
sirC
outlinks
bsu
BSU15630
BSU_15630
aminos
MLPLHISLEKKKVVIAGGGSIALRRLKTVISEGADITLVSPDVEPEIKQMAEERRIKWEKRTIEKEDYLNAFFIIAATDNAAVNKEIAQSASPFQLVNCVSDAELGNVYMPKIVKRGHVTVSVSTSGASPKHTKELAENVDKLIDGDFVAEVNRLYQMRRKK
[SW|Categories] containing this gene/protein
[SW|biosynthesis of cofactors]
Gene
Coordinates on the chromosome (coding sequence)
1,635,603 -> 1,636,091
Expression and Regulation
Operon
''[[protein|cysH]]-[[protein|cysP]]-[[protein|sat]]-[[protein|cysC]]-[[protein|ylnD]]-[[protein|ylnE]]-[[protein|ylnF]]'' [Pubmed|11004190]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|11004190]
Expression and Regulation
Regulation
repressed by casamino acids [Pubmed|12107147]
repressed in the presence of cysteine ([[protein|CymR]])
induced by methionine starvation ([[protein|S-box]]) [Pubmed|10094622]
Expression and Regulation
Regulatory mechanism
[[protein|S-box]]: transcription termination/ antitermination, the [[protein|S-box]] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622]
[[protein|CymR]]: transcription repression
References
proteinLength
162
geneLength
489
Gene
Coordinates
1,635,603 1,636,091
The protein
Catalyzed reaction/ biological activity
NAD+ + precorrin-2 --> 2 H+ + NADH + sirohydrochlorin (according to UniProt)
The protein
Protein family
ferrochelatase family (with [[protein|HemH]], according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|3DFZ] (36% identity) [pubmed|18588505]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B370 (ylnF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1369 NBRP B. subtilis, Japan]
BKE15630 ([[gene|ylnF]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCACAACCTTCTTCTTTT, downstream forward: _UP4_TAAGCCAGAGCATACAAATG
BKK15630 ([[gene|ylnF]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCACAACCTTCTTCTTTT, downstream forward: _UP4_TAAGCCAGAGCATACAAATG