Mon May 16 2016 17:11:38 GMT+0200 (CEST)2025-05-13 10:25:14
description
modulation of CheA activity in response to attractants, scaffold protein: facilitates coupling between CheA and receptors
modulation of [[protein|CheA]] activity in response to attractants, scaffold protein
locus
BSU16440
BSU_16440
function
control of CheA activity
control of [[protein|CheA]] activity
ec
outlinks
bsu
BSU16440
BSU_16440
[SW|Categories] containing this gene/protein
[SW|motility and chemotaxis]
Gene
Coordinates on the chromosome (coding sequence)
1,714,855 -> 1,715,325
Gene
Phenotypes of a mutant
''[[protein|cheV]] [[protein|cheW]]'' double mutants exhibit complete loss of chemotaxis [Pubmed|21098025]
''[[gene|cheV]] [[gene|cheW]]'' double mutants exhibit complete loss of chemotaxis [Pubmed|21098025]
The protein
Paralogous protein(s)
[[protein|CheV]] (N-terminal domain)
[[this]]
The protein
[SW|Localization]
cytoplasm (according to Swiss-Prot)
predominantly present at the cell poles [Pubmed|21098025]
cytoplasm (according to UniProt)
predominantly present at the cell poles [Pubmed|21098025]
The protein
[SW|Interactions]
[[protein|CheA]]-[[protein|CheW]] [Pubmed|25039821]
[[protein|McpB]]-[[protein|CheW]] [Pubmed|25039821]
The protein
Additional information
:
subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=
subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
Expression and Regulation
Operon
part of the [[fla-che operon]]
''[[protein|flgB]]-[[protein|flgC]]-[[protein|fliE]]-[[protein|fliF]]-[[protein|fliG]]-[[protein|fliH]]-[[protein|fliI]]-[[protein|fliJ]]-[[protein|ylxF]]-[[protein|fliK]]-[[protein|flgD]]-[[protein|flgE]]-[[protein|fliL]]-[[protein|fliM]]-[[protein|fliY]]-[[protein|cheY]]-[[protein|fliZ]]-[[protein|fliP]]-[[protein|fliQ]]-[[protein|fliR]]-[[protein|flhB]]-[[protein|flhA]]-[[protein|flhF]]-[[protein|flhG]]-[[protein|cheB]]-[[protein|cheA]]-[[protein|cheW]]-[[protein|cheC]]-[[protein|cheD]]-[[protein|sigD]]-[[protein|swrB]]'' [Pubmed|9657996,8157612,15175317]
''[[protein|ylxF]]-[[protein|fliK]]-[[protein|flgD]]-[[protein|flgE]]-[[protein|fliL]]-[[protein|fliM]]-[[protein|fliY]]-[[protein|cheY]]-[[protein|fliZ]]-[[protein|fliP]]-[[protein|fliQ]]-[[protein|fliR]]-[[protein|flhB]]-[[protein|flhA]]-[[protein|flhF]]-[[protein|flhG]]-[[protein|cheB]]-[[protein|cheA]]-[[protein|cheW]]-[[protein|cheC]]-[[protein|cheD]]-[[protein|sigD]]-[[protein|swrB]]'' [Pubmed|20233303]
Expression and Regulation
[SW|Sigma factor]
''[[protein|flgB]]'': [[protein|SigA]] [Pubmed|9657996], [[protein|SigD]] [Pubmed|9657996]
''[[protein|ylxF]]'': [[protein|SigD]] [Pubmed|20233303]
Expression and Regulation
Regulation
see [[fla-che operon]]
Expression and Regulation
Regulatory mechanism
see [[fla-che operon]]
Expression and Regulation
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=
in minimal medium, CheW is present with 2,100
proteinLength
156
geneLength
471
Gene
Coordinates
1,714,855 1,715,325
The protein
[SW|Domains]
CheW-like domain (aa 8-148) (according to UniProt)
The protein
Structure
[PDB|2HO9] (from E. coli, 28% identity)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE16440 ([[gene|cheW]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAGACACCTCCTCGA, downstream forward: _UP4_GCTTAATCTTAAAGGGGTTA
BKK16440 ([[gene|cheW]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAGACACCTCCTCGA, downstream forward: _UP4_GCTTAATCTTAAAGGGGTTA