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Mon May 16 2016 17:11:38 GMT+0200 (CEST)2025-05-13 10:25:14

description

modulation of CheA activity in response to attractants, scaffold protein: facilitates coupling between CheA and receptors

modulation of [[protein|CheA]] activity in response to attractants, scaffold protein

locus

BSU16440

BSU_16440

function

control of CheA activity

control of [[protein|CheA]] activity

ec

outlinks

bsu

BSU16440

BSU_16440

[SW|Categories] containing this gene/protein

[SW|motility and chemotaxis]

Gene

Coordinates on the chromosome (coding sequence)

1,714,855 -> 1,715,325

Gene

Phenotypes of a mutant

''[[protein|cheV]] [[protein|cheW]]'' double mutants exhibit complete loss of chemotaxis [Pubmed|21098025]

''[[gene|cheV]] [[gene|cheW]]'' double mutants exhibit complete loss of chemotaxis [Pubmed|21098025]

The protein

Paralogous protein(s)

[[protein|CheV]] (N-terminal domain)

[[this]]

The protein

[SW|Localization]

cytoplasm (according to Swiss-Prot)

predominantly present at the cell poles [Pubmed|21098025]

cytoplasm (according to UniProt)

predominantly present at the cell poles [Pubmed|21098025]

The protein

[SW|Interactions]

[[protein|CheA]]-[[protein|CheW]] [Pubmed|25039821]

[[protein|McpB]]-[[protein|CheW]] [Pubmed|25039821]

The protein

Additional information

:

subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17981983 PubMed]

subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operon

part of the [[fla-che operon]]

''[[protein|flgB]]-[[protein|flgC]]-[[protein|fliE]]-[[protein|fliF]]-[[protein|fliG]]-[[protein|fliH]]-[[protein|fliI]]-[[protein|fliJ]]-[[protein|ylxF]]-[[protein|fliK]]-[[protein|flgD]]-[[protein|flgE]]-[[protein|fliL]]-[[protein|fliM]]-[[protein|fliY]]-[[protein|cheY]]-[[protein|fliZ]]-[[protein|fliP]]-[[protein|fliQ]]-[[protein|fliR]]-[[protein|flhB]]-[[protein|flhA]]-[[protein|flhF]]-[[protein|flhG]]-[[protein|cheB]]-[[protein|cheA]]-[[protein|cheW]]-[[protein|cheC]]-[[protein|cheD]]-[[protein|sigD]]-[[protein|swrB]]'' [Pubmed|9657996,8157612,15175317]

''[[protein|ylxF]]-[[protein|fliK]]-[[protein|flgD]]-[[protein|flgE]]-[[protein|fliL]]-[[protein|fliM]]-[[protein|fliY]]-[[protein|cheY]]-[[protein|fliZ]]-[[protein|fliP]]-[[protein|fliQ]]-[[protein|fliR]]-[[protein|flhB]]-[[protein|flhA]]-[[protein|flhF]]-[[protein|flhG]]-[[protein|cheB]]-[[protein|cheA]]-[[protein|cheW]]-[[protein|cheC]]-[[protein|cheD]]-[[protein|sigD]]-[[protein|swrB]]'' [Pubmed|20233303]

Expression and Regulation

[SW|Sigma factor]

''[[protein|flgB]]'': [[protein|SigA]] [Pubmed|9657996], [[protein|SigD]] [Pubmed|9657996]

''[[protein|ylxF]]'': [[protein|SigD]] [Pubmed|20233303]

Expression and Regulation

Regulation

see [[fla-che operon]]

Expression and Regulation

Regulatory mechanism

see [[fla-che operon]]

Expression and Regulation

Additional information

subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17981983 PubMed]

in minimal medium, CheW is present with 2,100 /- 430 molecules per cell [PubMed|21515776]

proteinLength

156

geneLength

471

Gene

Coordinates

1,714,855 1,715,325

The protein

[SW|Domains]

CheW-like domain (aa 8-148) (according to UniProt)

The protein

Structure

[PDB|2HO9] (from E. coli, 28% identity)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE16440 ([[gene|cheW]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAGACACCTCCTCGA, downstream forward: _UP4_GCTTAATCTTAAAGGGGTTA

BKK16440 ([[gene|cheW]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAGACACCTCCTCGA, downstream forward: _UP4_GCTTAATCTTAAAGGGGTTA