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Fri Mar 11 2016 17:32:39 GMT+0100 (CET)2025-05-13 19:28:13

description

intramembrane metalloprotease, processing of pro-sigma-K to active SigK

intramembrane metalloprotease (site 2 protease), processing of pro-sigma-K to active [[protein|SigK]]

locus

BSU27970

BSU_27970

function

processing of pro-sigma-K to active SigK

processing of pro-sigma-K to active [[protein|SigK]]

ec

outlinks

bsu

BSU27970

BSU_27970

[SW|Categories] containing this gene/protein

[SW|sigma factors and their control], [SW|proteolysis], [SW|sporulation proteins], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence)

2,855,973 -> 2,856,839

The protein

Protein family

peptidase M50B family (according to Swiss-Prot)

[SW|peptidase M50B family] (according to UniProt)

The protein

[SW|Domains]

C-terminal cystathionine-beta-synthase (CBS) domain, this domain binds ATP [Pubmed|19805276]

C-terminal [SW|CBS domain], this domain binds ATP [Pubmed|19805276]

The protein

Effectors of protein activity

ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level [Pubmed|19805276]

ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level [Pubmed|19805276]

[[protein|SpoIVFB]] is kept inactive by the interaction with [[protein|SpoIVFA]] and [[protein|BofA]] [pubmed|15087499]

The protein

[SW|Localization]

integral membrane protein [Pubmed|11959848]

mother cell membrane [Pubmed|24243021]

mother cell integral membrane protein [Pubmed|24243021,11959848]

The protein

[SW|Interactions]

[SW|SpoIVFB]-pro[[protein|SigK]] [Pubmed|23995631,23585539,19805276]

[SW|SpoIVFB]-[[protein|BofA]], to inhibit proteolytic activity of [SW|SpoIVFB] [Pubmed|15087499]

[SW|SpoIVFB]-[SW|SpoIVFA], to facilitate the recruitment of [[protein|BofA]] to [SW|SpoIVFB] [Pubmed|11959848]

Expression and Regulation

Operon

''[SW|spoIVFA]-[SW|spoIVFB]'' [Pubmed|1942049]

''[SW|spoIVFA]-[SW|spoIVFB]-[[protein|rplU]]-[[protein|prp]]-[[protein|rpmA]]'' [Pubmed|22383849]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigE]] [Pubmed|15699190,1942049]

Expression and Regulation

Regulation

expressed early during sporulation in the mother cell ([[protein|SigE]], [SW|SpoIIID]) [Pubmed|15699190,1942049,15383836]

Expression and Regulation

Regulatory mechanism

[SW|SpoIIID]: transcription repression [Pubmed|15383836]

References

Reviews

20836086, 23479438, 19189971, 24099006

20836086, 23479438, 19189971, 24099006, 31350897

References

Original Publications

11959848, 9501233, 12940997, 1577688, 12060714, 9078383, 1942049, 10611287, 15383836, 16818230, 19805276, 15699190, 23585539, 23995631, 15087499, 24243021, 22383849

proteinLength

288

geneLength

867

Gene

Coordinates

2,855,973 2,856,839

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE27970 ([[gene|spoIVFB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA, downstream forward: _UP4_TAAAACTGATTGACAAACGC

BKK27970 ([[gene|spoIVFB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA, downstream forward: _UP4_TAAAACTGATTGACAAACGC

References

Original Publication

11959848, 9501233, 12940997, 1577688, 12060714, 9078383, 1942049, 10611287, 15383836, 16818230, 19805276, 15699190, 23585539, 23995631, 15087499, 24243021, 22383849, 26953342, 29180425, 30403663, 32515031