Fri Mar 11 2016 17:32:39 GMT+0100 (CET)2025-05-13 19:28:13
description
intramembrane metalloprotease, processing of pro-sigma-K to active SigK
intramembrane metalloprotease (site 2 protease), processing of pro-sigma-K to active [[protein|SigK]]
locus
BSU27970
BSU_27970
function
processing of pro-sigma-K to active SigK
processing of pro-sigma-K to active [[protein|SigK]]
ec
outlinks
bsu
BSU27970
BSU_27970
[SW|Categories] containing this gene/protein
[SW|sigma factors and their control], [SW|proteolysis], [SW|sporulation proteins], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
2,855,973 -> 2,856,839
The protein
Protein family
peptidase M50B family (according to Swiss-Prot)
[SW|peptidase M50B family] (according to UniProt)
The protein
[SW|Domains]
C-terminal cystathionine-beta-synthase (CBS) domain, this domain binds ATP [Pubmed|19805276]
C-terminal [SW|CBS domain], this domain binds ATP [Pubmed|19805276]
The protein
Effectors of protein activity
ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level [Pubmed|19805276]
ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level [Pubmed|19805276]
[[protein|SpoIVFB]] is kept inactive by the interaction with [[protein|SpoIVFA]] and [[protein|BofA]] [pubmed|15087499]
The protein
[SW|Localization]
integral membrane protein [Pubmed|11959848]
mother cell membrane [Pubmed|24243021]
mother cell integral membrane protein [Pubmed|24243021,11959848]
The protein
[SW|Interactions]
[SW|SpoIVFB]-pro[[protein|SigK]] [Pubmed|23995631,23585539,19805276]
[SW|SpoIVFB]-[[protein|BofA]], to inhibit proteolytic activity of [SW|SpoIVFB] [Pubmed|15087499]
[SW|SpoIVFB]-[SW|SpoIVFA], to facilitate the recruitment of [[protein|BofA]] to [SW|SpoIVFB] [Pubmed|11959848]
Expression and Regulation
Operon
''[SW|spoIVFA]-[SW|spoIVFB]'' [Pubmed|1942049]
''[SW|spoIVFA]-[SW|spoIVFB]-[[protein|rplU]]-[[protein|prp]]-[[protein|rpmA]]'' [Pubmed|22383849]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigE]] [Pubmed|15699190,1942049]
Expression and Regulation
Regulation
expressed early during sporulation in the mother cell ([[protein|SigE]], [SW|SpoIIID]) [Pubmed|15699190,1942049,15383836]
Expression and Regulation
Regulatory mechanism
[SW|SpoIIID]: transcription repression [Pubmed|15383836]
References
Reviews
References
Original Publications
proteinLength
288
geneLength
867
Gene
Coordinates
2,855,973 2,856,839
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE27970 ([[gene|spoIVFB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA, downstream forward: _UP4_TAAAACTGATTGACAAACGC
BKK27970 ([[gene|spoIVFB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA, downstream forward: _UP4_TAAAACTGATTGACAAACGC
References
Original Publication