SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-05-29 08:47:42

locus

BSU11670

BSU_11670

outlinks

bsu

BSU11670

BSU_11670

aminos

MKRHYEAVVIGGGIIGSAIAYYLAKENKNTALFESGTMGGRTTSAAAGMLGAHAECEERDAFFDFAMHSQRLYKGLGEELYALSGVDIRQHNGGMFKLAFSEEDVLQLRQMDDLDSVSWYSKEEVLEKEPYASGDIFGASFIQDDVHVEPYFVCKAYVKAAKMLGAEIFEHTPVLHVERDGEALFIKTPSGDVWANHVVVASGVWSGMFFKQLGLNNAFLPVKGECLSVWNDDIPLTKTLYHDHCYIVPRKSGRLVVGATMKPGDWSETPDLGGLESVMKKAKTMLPAIQNMKVDRFWAGLRPGTKDGKPYIGRHPEDSRILFAAGHFRNGILLAPATGALISDLIMNKEVNQDWLHAFRIDRKEAVQI

[SW|Categories] containing this gene/protein

[SW|biosynthesis of cofactors]

Gene

Coordinates on the chromosome (coding sequence)

1,243,735 -> 1,244,844

The protein

Catalyzed reaction/ biological activity

Glycine + H2O + O2 = glyoxylate + NH3 + H2O2 (according to Swiss-Prot)

glycine + H2O + O2 --> glyoxylate + H2O2 + NH4+ (according to UniProt)

H2O + N-ethylglycine + O2 --> ethylamine + glyoxylate + H2O2 (according to UniProt)

H2O + O2 + sarcosine --> glyoxylate + H2O2 + methylamine (according to UniProt)

D-alanine + H2O + O2 --> H2O2 + NH4+ + pyruvate (according to UniProt)

glyphosate + H2O + O2 --> aminomethylphosphonate + glyoxylate + H+ + H2O2 (according to UniProt)

The protein

Protein family

DAMOX/DASOX family (according to Swiss-Prot)

DAO family (single member, according to UniProt)

The protein

[SW|Interactions]

the FAD-containing holoenzyme is a homotetramer [Pubmed|19751796]

Expression and Regulation

Operon

''[[protein|tenA]]-[[protein|tenI]]-[[protein|thiO]]-[[protein|thiS]]-[[protein|thiG]]-[[protein|thiF]]-[[protein|thiD]]'' [Pubmed|1898926]

Expression and Regulation

Regulation

repressed by thiamine ([[protein|Thi-box]]) [Pubmed|16356850]

Expression and Regulation

Regulatory mechanism

[[protein|Thi-box]]: [SW|RNA switch] [Pubmed|16356850]

References

Original publications

19254749, 11744710, 12654003, 9827558, 12627963, 15105420, 19751796, 19864430, 14567704, 20690620, 24925096

19254749, 11744710, 12654003, 9827558, 12627963, 15105420, 19751796, 19864430, 14567704, 20690620, 24925096, 31562850

proteinLength

369

geneLength

1110

Gene

Coordinates

1,243,735 1,244,844

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Cofactors]

FAD (according to UniProt)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-B164 (yjbR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1163 NBRP B. subtilis, Japan]

BKE11670 ([[gene|thiO]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTCCGCCTCCAATCACCA, downstream forward: _UP4_CGCAAGGAGGCGGTTCAGAT

BKK11670 ([[gene|thiO]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTCCGCCTCCAATCACCA, downstream forward: _UP4_CGCAAGGAGGCGGTTCAGAT