2017-05-02 13:23:132025-05-23 11:36:27
description
AAA unfoldase, ATPase subunit of the ClpC-ClpP protease, directs proteins phosphorylated on arginine residues to ClpP
AAA unfoldase, ATPase subunit of the [[protein|ClpC]]-[[protein|ClpP]] protease, directs proteins phosphorylated on arginine residues to [[protein|ClpP]]
locus
BSU00860
BSU_00860
geneLength
2430
2433
function
protein degradationpositive regulator of autolysin (LytC and LytD) synthesis
protein degradation, positive regulator of autolysin ([[protein|LytC]] and [[protein|LytD]]) synthesis
product
AAA unfoldase, ATPase subunit of the ClpC-ClpP protease
AAA unfoldase, ATPase subunit of the [[protein|ClpC]]-[[protein|ClpP]] protease
outlinks
bsu
BSU00860
BSU_00860
Gene
Coordinates
103,572 → 106,004
103,572 106,004
The protein
Protein family
[[gene|mecA]] family (according to Swiss-Prot) clpA/clpB family. [[protein|ClpC]] subfamily (according to Swiss-Prot), AAA -type ATPase (IPR013093) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR013093 InterPro] (PF07724) [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]
ClpA/ClpB family (with [[protein|ClpE]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|ClpE]], [[protein|ClpX]]
[[this]]
The protein
[SW|Domains]
AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]
AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]
[SW|UVR domain] (aa 417-452) (according to UniProt)
The protein
Modification
phosphorylated on Arg-5 and Arg-254 [Pubmed|22517742]
phosphorylated on Arg-5 and Arg-254 [Pubmed|22517742]
phosphorylated on Arg-70 [Pubmed|31221751]
The protein
Structure
[PDB|2K77] (N-terminal domain)
[PDB|3PXG], [PDB|3J3U 3J3U] (the [[protein|MecA]]-[[protein|ClpC]] complex) [Pubmed|23595989]
[PDB|2K77] (N-terminal domain)
[PDB|3PXG], [PDB|3J3U] (the [[protein|MecA]]-[[protein|ClpC]] complex) [Pubmed|23595989]
The protein
[SW|Localization]
cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heatshock, colocalization with [[protein|ClpP]] [http://www.ncbi.nlm.nih.gov/pubmed/18786145 Pubmed]
forms foci coincident with nucleoid edges, usually near cell poles [Pubmed|18689473]
[[File:ClpC.jpg ]]
cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heatshock, colocalization with [[protein|ClpP]] [http://www.ncbi.nlm.nih.gov/pubmed/18786145 Pubmed]
forms foci coincident with nucleoid edges, usually near cell poles [Pubmed|18689473]
Biological materials
Mutant
[[gene|clpC]]::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab
BP98 (''clpC''::''spc''), available in [SW|Fabian Commichau]'s lab [Pubmed|25610436]
BKE00860 (''clpC''::''erm trpC2'') is available from the [http://bgsc.org Bacillus Genetic Stock Center]
[[gene|clpC]]::tet available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen] Lab
BP98 (''clpC''::''spc''), available in [SW|Fabian Commichau]'s lab [Pubmed|25610436]
BKE00860 ([[gene|clpC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCGTTCTGTAAATCTTCCAA, downstream forward: _UP4_TAATATAGAAGACGGAAATG
BKK00860 ([[gene|clpC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCGTTCTGTAAATCTTCCAA, downstream forward: _UP4_TAATATAGAAGACGGAAATG
Labs working on this gene/protein
[SW|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]
[SW|Kürsad Turgay], Freie Universität Berlin, Germany [http://www.biologie.fu-berlin.de/en/microbio/mibi-turg/index.html homepage]
References
Reviews
References
Original Publications
labs
[SW|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]
[SW|Kürsad Turgay], Freie Universitt Berlin, Germany [http://www.biologie.fu-berlin.de/en/microbio/mibi-turg/index.html homepage]