Tue Dec 13 2016 11:16:36 GMT+0100 (CET)2025-05-10 08:36:05
description
carboxy-terminal processing serine protease, cleaves SpoIVFA, this results in processing of pro-SigK
carboxy-terminal processing serine protease, cleaves [[protein|SpoIVFA]], this results in processing of pro-[[protein|SigK]]
locus
BSU35240
BSU_35240
function
control of SigK activation
control of [[protein|SigK]] activation
product
carboxy-terminal processing serine protease,
cleaves SpoIVFA
carboxy-terminal processing serine protease, cleaves [[protein|SpoIVFA]]
ec
outlinks
bsu
BSU35240
BSU_35240
aminos
MNQKIMAVIAAGSMLFGGAGVYAGINLLEMDKPQTAAVPATAQADSERDKAMDKIEKAYELISNEYVEKVDREKLLEGAIQGMLSTLNDPYSVYMDKQTAKQFSDSLDSSFEGIGAEVGMEDGKIIIVSPFKKSPAEKAGLKPNDEIISINGESMAGKDLNHAVLKIRGKKGSSVSMKIQRPGTKKQLSFRIKRAEIPLETVFASEKKVQGHSVGYIAISTFSEHTAEDFAKALRELEKKEIEGLVIDVRGNPGGYLQSVEEILKHFVTKDQPYIQIAERNGDKKRYFSTLTHKKAYPVNVITDKGSASASEILAGALKEAGHYDVVGDTSFGKGTVQQAVPMGDGSNIKLTLYKWLTPNGNWIHKKGIEPTIAIKQPDYFSAGPLQLKEPLKVDMNNEDVKHAQVLLKGLSFDPGREDGYFSKDMKKAVMAFQDQNKLNKTGVIDTRTAETLNQQIEKKKSDEKNDLQLQTALKSLFVN
[SW|Categories] containing this gene/protein
[SW|proteolysis], [SW|sigma factors and their control], [SW|sporulation proteins]
Gene
Coordinates on the chromosome (coding sequence)
3,622,356 -> 3,623,798
The protein
Protein family
PDZ protease [Pubmed|24243021]
Peptidase s41a family (together with [[protein|CtpA]], according to Uniprot)
PDZ protease [Pubmed|24243021]
The protein
Paralogous protein(s)
[[protein|CtpA]]
[[this]]
The protein
Effectors of protein activity
substrate binding of CtpB induces opening of the PDZ gate and protease activation [Pubmed|24243021]
[[protein|CtpB]] acts in concert with a second signaling protease, [SW|SpoIVB] [Pubmed|24243021]
substrate binding of CtpB induces opening of the PDZ gate and protease activation [Pubmed|24243021]
[[protein|CtpB]] acts in concert with a second signaling protease, [[protein|SpoIVB]] [Pubmed|24243021]
The protein
[SW|Interactions]
forms a dimeric ring [Pubmed|24243021]
[[protein|CtpB]]-[SW|SpoIVFA] [Pubmed|24243021]
Expression and Regulation
Operon
''ctpB'' (according to [http://dbtbs.hgc.jp/COG/prom/ctpB.html DBTBS])
Expression and Regulation
[SW|Sigma factor]
[[protein|SigE]] [Pubmed|15699190], [[protein|SigG]] [Pubmed|15699190,16497325]
Expression and Regulation
Regulation
expressed during sporulation ([[protein|SigE]], [[protein|SigG]]) [Pubmed|15699190,16497325]
Biological materials
Mutant
1S129 ( ''ctpB''::''tet''), [Pubmed|14526016], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S129&Search=1S129 BGSC]
MGNA-A074 (yvjB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/74 NBRP B. subtilis, Japan]
1S129 ( ''ctpB''::''tet''), [Pubmed|14526016], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S129&Search=1S129 BGSC]
BKE35240 ([[gene|ctpB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTTTACTCCTCCTGTA, downstream forward: _UP4_TAAAGGAGATGGTATTTCAT
BKK35240 ([[gene|ctpB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTTTACTCCTCCTGTA, downstream forward: _UP4_TAAAGGAGATGGTATTTCAT
References
proteinLength
480
geneLength
1443
Gene
Coordinates
3,622,356 3,623,798
The protein
Catalyzed reaction/ biological activity
The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala (according to UniProt)
The protein
[SW|Domains]
signal peptide (aa 1 - 23) (according to Uniprot)
[SW|PDZ domain] (aa 92 - 182) (according to Uniprot)
S41 peptidase domain (by similarity to [[protein|CtpA]]) [pubmed|29979679]
peptidoglycan binding domain (C-terminal) (by similarity to [[protein|CtpA]]) [pubmed|29979679]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
References
Reviews
References
Original Publications