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Mon Jul 04 2016 16:11:48 GMT+0200 (CEST)2025-05-22 12:24:15

description

DNA polymerase III (beta subunit), beta clamp, part of the replisome, also involved in DNA mismatch repair, inhibitor of DnaA oligomerization

DNA polymerase III (beta subunit), beta clamp, part of the [SW|replisome], also involved in DNA mismatch repair, inhibitor of [[protein|DnaA]] oligomerization

locus

BSU00020

BSU_00020

function

DNA replication, DNA repair

[[category|DNA replication]], DNA repair

product

DNA polymerase III
(beta subunit), beta clamp

DNA polymerase III (beta subunit), beta clamp

outlinks

bsu

BSU00020

BSU_00020

[SW|Categories] containing this gene/protein

[SW|DNA replication], [SW|essential genes]

Gene

Coordinates on the chromosome (coding sequence)

1,939 -> 3,075

The protein

Catalyzed reaction/ biological activity

[[protein|DnaN]] overexpression or release from the [SW|replisome] decreases association of [[protein|YabA]] with ''oriC'', increases association of [[protein|DnaA]] with ''oriC'' [Pubmed|21895792]

Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n 1) (according to Swiss-Prot)

recruits [[protein|MutS]] to the site of [SW|DNA replication] ([SW|replisome]) [Pubmed|23228104]

required for bacteriophage SPP1 replication [Pubmed|23268446]

inhibits oligomerization and helix formation of [[protein|DnaA]] [Pubmed|23909787]

[[protein|DnaN]] overexpression or release from the [SW|replisome] decreases association of [[protein|YabA]] with ''oriC'', increases association of [[protein|DnaA]] with ''oriC'' [Pubmed|21895792]

recruits [[protein|MutS]] to the site of [SW|DNA replication] ([SW|replisome]) [Pubmed|23228104]

required for bacteriophage SPP1 replication [Pubmed|23268446]

inhibits oligomerization and helix formation of [[protein|DnaA]] [Pubmed|23909787]

The protein

Structure

[PDB|2AWA] (from ''Streptococcus pneumoniae'', 41% identity, 77% similarity)

[PDB|4TR6] [pubmed|25170813]

[PDB|6E8D] (complex with [[protein|MutL]]) [Pubmed|30916336]

The protein

[SW|Localization]

nucleoid (mid-cell spot) [Pubmed|9822387] [Pubmed|16479537]

forms large assemblies on DNA, called clamp zones [Pubmed|21419346]

forms foci [Pubmed|21958350]

nucleoid (mid-cell spot) [Pubmed|9822387] [Pubmed|16479537]

forms large assemblies on DNA, called clamp zones [Pubmed|21419346]

forms foci [Pubmed|21958350]

The protein

[SW|Interactions]

[[protein|DnaA]]-[[protein|YabA]]-[[protein|DnaN]] [Pubmed|12060778]

part of the [SW|replisome]: [[protein|PolC]]-[[protein|HolA]]-[[protein|HolB]]-[[protein|DnaX]]-[[protein|DnaN]]-[[protein|DnaG]]-[[protein|DnaC]]-[[protein|DnaI]]-[[protein|DnaD]]-[[protein|SsbA]]-[[protein|DnaE]]-[[protein|PriA]]-[[protein|DnaB]] [Pubmed|20122408]

[SW|PolY1]-[[protein|DnaN]], [SW|PolY2]-[[protein|DnaN]] (required for untargeted mutagenesis) [Pubmed|15469515]

[SW|PolY1]-[[protein|PolA]]-[[protein|DnaN]] [Pubmed|16045613]

[[protein|MutL]] (C-terminal domain)-[[protein|DnaN]], the interaction stimulates the endonuclease activity of [SW|MutL] [Pubmed|26384423,21050827]

[[protein|MutS]]-[[protein|DnaN]] [Pubmed|20453097]

[[protein|DnaE]]-[[protein|DnaN]] [Pubmed|21958350]

[[protein|DnaN]] forms dimers [Pubmed|16045613]

Expression and Regulation

Operon

''[[protein|dnaA]]-[[protein|dnaN]]'' [Pubmed|2987848]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|2987848]

Expression and Regulation

Regulation

negatively controlled by [[protein|DnaA]] [Pubmed|2168872] and [SW|Spo0A] [Pubmed|14651647]

repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Expression and Regulation

Regulatory mechanism

[SW|Spo0A]: transcription repression [Pubmed|14651647]

References

Reviews

20157337, 22933559

20157337, 22933559, 26343983, 28075389

References

Original publications

16942601, 9822387, 14651647, 2987848, 2168872, 14651647, 12060778, 16461910, 16479537, 19737352, 19081080, 11395445, 20122408, 20451384, 15469515, 16045613, 21895792, 20453097, 23228104, 23268446, 21050827, 21419346, 21958350, 23909787, 25170813, 26384423, 26615189, 27369079

16942601, 9822387, 14651647, 2987848, 2168872, 14651647, 12060778, 16461910, 16479537, 19737352, 19081080, 11395445, 20122408, 20451384, 15469515, 16045613, 21895792, 20453097, 23228104, 23268446, 21050827, 21419346, 21958350, 23909787, 25170813, 26384423, 26615189, 27369079, 28878042, 25170813, 30391220, 30916336

proteinLength

378

geneLength

1137

Gene

Coordinates

1,939 3,075

The protein

Protein family

Beta sliding clamp family (single member, according to UniProt)

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]