Tue Dec 13 2016 11:16:31 GMT+0100 (CET)2025-03-29 16:28:33
title
yfnA
mtrA
description
similar to metabolite transport protein
methylthioribose transporter
locus
BSU07340
BSU_07340
function
unknown
uptake of methylthioribose
product
unknown
methylthioribose transporter
ec
names
yfnA
mtrA
yfnA
outlinks
bsu
BSU07340
BSU_07340
aminos
MSSLFRKKPLETLSAQSKSKSLARTLSAFDLTLLGIGCVIGTGIFVITGTVAATGAGPALIISFILAGLACALAAFCYAEFSSSIPISGSVYSYSYVTLGELLAFLIGWDLMLEYVIALSAVATGWSSYFQSLLAGFNLHIPAALTGAPGSMAGAVFNLPAAVIILLITAIVSRGVKESTRFNNVIVLMKIAIILLFIIVGIGYVKPDNWSPFMPFGMKGVILSAATVFFAYLGFDAVSNASEEVKNPQKNMPVGIISALAVCTVLYIAVSLVLTGMMPYAKLNVGDPVSFALKFVGQDAVAGIISVGAIIGITTVMLALLYAQVRLTFAMSRDGLLPGLFAKVHPSFKTPFRNTWLTGIVAAGIAGFINLGTLAHLVNMGTLAAFTVISIAVIVLRKKHPEIKASFRVPFVPVVPIISAGICLWFMYSLPGVTWLSFVIWIAVGTLVYFLYSRKHSLLNK
[SW|Categories] containing this gene/protein
[SW|transporters/ other], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
805,456 -> 806,841
The protein
Paralogous protein(s)
[[protein|BcaP]]
[[this]]
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot), membrane associated [Pubmed|18763711]
cell membrane [Pubmed|18763711]
References
proteinLength
461
geneLength
1386
Gene
Coordinates
805,456 806,841
The protein
Catalyzed reaction/ biological activity
uptake of methylthioribose [pubmed|29280348]
The protein
Protein family
[SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
The protein
Structure
[PDB|5OQT] (from Geobacillus kaustophilus, 60% identity) [pubmed|29416041]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
GP2379 ∆''[[gene|mtrA]]''::''kan'', available in [SW|Jörg Stülke]'s lab [pubmed|32743959]
MGNA-C231 (yfnA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2229 NBRP B. subtilis, Japan]
BKE07340 ([[gene|mtrA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAAAGTTCCTCCTAGA, downstream forward: _UP4_TAATCTCTTTTCAGCCGGCG
BKK07340 ([[gene|mtrA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAAAGTTCCTCCTAGA, downstream forward: _UP4_TAATCTCTTTTCAGCCGGCG
Biological materials
Expression vector
pGP2281: expression of ''[[gene|mtrA]]'' by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
Biological materials
lacZ fusion
pGP2278 (in [SW|pAC5]) (GP2964), available in [SW|Jörg Stülke]'s lab