SubtiBank SubtiBank
Version comparison:

2018-07-26 15:51:122025-05-24 13:53:28

locus

BSU07700

BSU_07700

geneLength

1356

1359

outlinks

bsu

BSU07700

BSU_07700

Gene

Coordinates

840,656 → 842,014

840,656 842,014

The protein

Catalyzed reaction/ biological activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

The protein

[SW|Localization]

cell membrane (according to Swiss-Prot), membrane associated [Pubmed|18763711]

cell membrane [Pubmed|18763711]

Biological materials

Mutant

MGNA-C339 (yflF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2337 NBRP B. subtilis, Japan]

BKE07700 (Δ[[gene|nagP]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCCATCCCCCTCATAC, downstream forward: _UP4_TAAAAAAGCGGAGAGGGCAA

BKK07700 (Δ[[gene|nagP]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCCATCCCCCTCATAC, downstream forward: _UP4_TAAAAAAGCGGAGAGGGCAA

MGNA-C339 (yflF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2337 NBRP B. subtilis, Japan]

BKE07700 ([[gene|nagP]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCCATCCCCCTCATAC, downstream forward: _UP4_TAAAAAAGCGGAGAGGGCAA

BKK07700 ([[gene|nagP]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07700 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCCATCCCCCTCATAC, downstream forward: _UP4_TAAAAAAGCGGAGAGGGCAA

References

Original publications

10627040, 18763711, 10627040, 6174502, 23667565, 21602348, 23876412, 30038046

10627040, 18763711, 10627040, 6174502, 23667565, 21602348, 23876412, 30038046, 29784777

The protein

[SW|Domains]

[SW|PTS EIIB domain] type-1 (aa 375-452) (according to UniProt)

[SW|PTS EIIC domain] type-1 (aa 1-361) (according to UniProt)

The protein

Structure

[PDB|6BVG] (from B. cereus, the EIIC domain, corresponds to aa 3 ... 362 of NagP, 28% identity) [pubmed|29784777]