Mon Aug 31 2015 13:56:08 GMT+0200 (CEST)2025-05-14 13:47:36
locus
BSU28560
BSU_28560
outlinks
bsu
BSU28560
BSU_28560
syns
[SW|Categories] containing this gene/protein
[SW|utilization of lipids]
The protein
Catalyzed reaction/ biological activity
ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]
long-chain fatty acid + ATP + CoA --> long-chain fatty acyl-CoA + AMP + diphosphate (according to UniProt)
activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]
The protein
Protein family
ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
[SW|ATP-dependent AMP-binding enzyme family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|LcfB]], [[protein|YhfT]], [[protein|YngI]]
[[this]]
Expression and Regulation
Operon
''[[protein|lcfA]]-[[protein|fadR]]-[[protein|fadB]]-[[protein|etfB]]-[[protein|etfA]]'' [Pubmed|17189250]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|21398533]
Expression and Regulation
Regulation
subject to carbon catabolite repression ([[protein|CcpA]]-[[PtsH|HPr(Ser-P)]] [Pubmed|21398533]
induced by long chain acyl-CoA (C14 ... C20) ([[protein|FadR]]) [Pubmed|17189250]
Expression and Regulation
Regulatory mechanism
[[protein|CcpA]]-[[PtsH|HPr(Ser-P)]]: transcription repression [Pubmed|21398533]
[[protein|FadR]]: transcription repression [Pubmed|17189250]
Biological materials
Mutant
available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]
1A852 ( ''lcfA''::''cat''), [Pubmed|17085570], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A852&Search=1A852 BGSC]
available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]
1A852 ( ''lcfA''::''cat''), [Pubmed|17085570], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A852&Search=1A852 BGSC]
BKE28560 ([[gene|lcfA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA, downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT
BKK28560 ([[gene|lcfA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA, downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT
References
proteinLength
560
geneLength
1683
Gene
Coordinates
2,918,646 2,920,328
The protein
Structure
[PDB|3R44] (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 28% identity) [pubmed|22560731]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]