SubtiBank SubtiBank
Version comparison:

Mon Aug 31 2015 13:56:08 GMT+0200 (CEST)2025-05-14 13:47:36

locus

BSU28560

BSU_28560

outlinks

bsu

BSU28560

BSU_28560

syns

[SW|Categories] containing this gene/protein

[SW|utilization of lipids]

The protein

Catalyzed reaction/ biological activity

ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]

long-chain fatty acid + ATP + CoA --> long-chain fatty acyl-CoA + AMP + diphosphate (according to UniProt)

activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]

The protein

Protein family

ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

[SW|ATP-dependent AMP-binding enzyme family] (according to UniProt)

The protein

Paralogous protein(s)

[[protein|LcfB]], [[protein|YhfT]], [[protein|YngI]]

[[this]]

Expression and Regulation

Operon

''[[protein|lcfA]]-[[protein|fadR]]-[[protein|fadB]]-[[protein|etfB]]-[[protein|etfA]]'' [Pubmed|17189250]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|21398533]

Expression and Regulation

Regulation

subject to carbon catabolite repression ([[protein|CcpA]]-[[PtsH|HPr(Ser-P)]] [Pubmed|21398533]

induced by long chain acyl-CoA (C14 ... C20) ([[protein|FadR]]) [Pubmed|17189250]

Expression and Regulation

Regulatory mechanism

[[protein|CcpA]]-[[PtsH|HPr(Ser-P)]]: transcription repression [Pubmed|21398533]

[[protein|FadR]]: transcription repression [Pubmed|17189250]

Biological materials

Mutant

available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]

1A852 ( ''lcfA''::''cat''), [Pubmed|17085570], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A852&Search=1A852 BGSC]

available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]

1A852 ( ''lcfA''::''cat''), [Pubmed|17085570], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A852&Search=1A852 BGSC]

BKE28560 ([[gene|lcfA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA, downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT

BKK28560 ([[gene|lcfA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA, downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT

References

17189250, 17919287, 20797616, 17085570, 22900538

17189250, 17919287, 20797616, 17085570, 22900538, 22560731, 31113899

proteinLength

560

geneLength

1683

Gene

Coordinates

2,918,646 2,920,328

The protein

Structure

[PDB|3R44] (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 28% identity) [pubmed|22560731]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]