Tue Dec 13 2016 11:16:32 GMT+0100 (CET)2025-05-11 01:18:19
description
transcription repressor of the ohrA gene
transcription repressor of the [[gene|ohrA]] gene
locus
BSU13150
BSU_13150
function
regulation of ohrA expression
in response to organic peroxides
regulation of [[gene|ohrA]] expression in response to organic peroxides
product
transcription repressor (MarR family)
transcription repressor ([SW|MarR family])
ec
outlinks
bsu
BSU13150
BSU_13150
aminos
MENKFDHMKLENQLCFLLYASSREMTKQYKPLLDKLNITYPQYLALLLLWEHETLTVKKMGEQLYLDSGTLTPMLKRMEQQGLITRKRSEEDERSVLISLTEDGALLKEKAVDIPGTILGLSKQSGEDLKQLKSALYTLLETLHQKN
[SW|Categories] containing this gene/protein
[SW|transcription factors and their control], [SW|resistance against oxidative and electrophile stress]
Gene
Coordinates on the chromosome (coding sequence)
1,381,434 -> 1,381,877
The protein
Modification
senses organic peroxides and NaOCl, released from DNA upon oxidation of an active site cysteine
S-bacillithiolated by NaOCl and CHP stress on Cys-15, this results in release from the'' [[protein|ohrA]]'' promoter [Pubmed|21749987]
senses organic peroxides and NaOCl, released from DNA upon oxidation of an active site cysteine
S-bacillithiolated by NaOCl and CHP stress on Cys-15, this results in release from the'' [[gene|ohrA]]'' promoter [Pubmed|21749987]
The protein
Structure
[PDB|1Z91] (reduced form), [PDB|1Z9C] (complex with ''[[protein|ohrA]]'' promoter)
[PDB|1Z91] (reduced form), [PDB|1Z9C] (complex with ''[[gene|ohrA]]'' promoter)
The protein
[SW|Interactions]
forms dimers
[[protein|BrxA]]-[[protein|OhrR]], to de-bacillithiolate S-bacillithiolated [[protein|OhrR]] [Pubmed|24313874]
[[protein|BrxB]]-[[protein|OhrR]], to de-bacillithiolate S-bacillithiolated [[protein|OhrR]] [Pubmed|24313874]
Expression and Regulation
Operon
''ohrR'' [Pubmed|9696771]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]]
Labs working on this gene/protein
[SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
[SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
proteinLength
147
geneLength
444
Gene
Coordinates
1,381,434 1,381,877
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|HTH marR-type domain] (aa 11-141) (according to UniProt)
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
GP3172 [[gene|ohrR]]::kan trpC2 available in [SW|Jörg Stülke]'s lab
MGNA-A750 (ykmA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/750 NBRP B. subtilis, Japan]
BKE13150 ([[gene|ohrR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTCACCAACTCTT, downstream forward: _UP4_TGAGGATGCCTTGTACAGGC
BKK13150 ([[gene|ohrR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTCACCAACTCTT, downstream forward: _UP4_TGAGGATGCCTTGTACAGGC
labs
[SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
[SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]