2017-08-28 08:32:582025-05-14 22:26:14
title
ycsF
pxpA
description
similar to lactam utilization protein
subunit of ATP-dependent 5-oxoprolinase
locus
BSU04050
BSU_04050
geneLength
771
774
function
unknown
detoxification of 5-oxoproline
product
unknown
subunit of 5-oxoprolinase
names
ycsF
pxpA
ycsF
outlinks
bsu
BSU04050
BSU_04050
Gene
Coordinates
457,023 → 457,796
457,023 457,796
The protein
Protein family
UPF0271 (lamB) family (according to Swiss-Prot)
LamB/PxpA family (single member, according to UniProt)
The protein
Structure
[PDB|1V6T] (from ''Pyrococcus Horikoshii'', 52% identity)
[PDB|1V6T] (from ''Pyrococcus horikoshii'', 52% identity)
Biological materials
Mutant
MGNA-C072 (ycsF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2070 NBRP B. subtilis, Japan]
MGNA-C072 (ycsF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2070 NBRP B. subtilis, Japan]
BKE04050 ([[gene|pxpA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTGCCAA, downstream forward: _UP4_TCAACATAAAGGAGGAACAA
BKK04050 ([[gene|pxpA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTGCCAA, downstream forward: _UP4_TCAACATAAAGGAGGAACAA
References
Gene
Phenotypes of a mutant
no growth with 5-oxoproline as single source of nitrogen [pubmed|28830929]
reduced growth with ammonium as source of nitrogen due to the accumulation of toxic 5-oxoproline [pubmed|28830929]
The protein
Catalyzed reaction/ biological activity
5-oxoproline + ATP --> glutamate + ADP + Pi [pubmed|28830929]
The protein
Paralogous protein(s)
[[this]]
The protein
Kinetic information
Km (5-oxoproline): 39 M [pubmed|28830929]