SubtiBank SubtiBank
Version comparison:

2017-08-28 08:32:582025-05-14 22:26:14

title

ycsF

pxpA

description

similar to lactam utilization protein

subunit of ATP-dependent 5-oxoprolinase

locus

BSU04050

BSU_04050

geneLength

771

774

function

unknown

detoxification of 5-oxoproline

product

unknown

subunit of 5-oxoprolinase

names

ycsF

pxpA

ycsF

outlinks

bsu

BSU04050

BSU_04050

Gene

Coordinates

457,023 → 457,796

457,023 457,796

The protein

Protein family

UPF0271 (lamB) family (according to Swiss-Prot)

LamB/PxpA family (single member, according to UniProt)

The protein

Structure

[PDB|1V6T] (from ''Pyrococcus Horikoshii'', 52% identity)

[PDB|1V6T] (from ''Pyrococcus horikoshii'', 52% identity)

Biological materials

Mutant

MGNA-C072 (ycsF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2070 NBRP B. subtilis, Japan]

MGNA-C072 (ycsF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2070 NBRP B. subtilis, Japan]

BKE04050 ([[gene|pxpA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTGCCAA, downstream forward: _UP4_TCAACATAAAGGAGGAACAA

BKK04050 ([[gene|pxpA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTGCCAA, downstream forward: _UP4_TCAACATAAAGGAGGAACAA

References

9334321, 25755103

9334321, 25755103, 28830929

Gene

Phenotypes of a mutant

no growth with 5-oxoproline as single source of nitrogen [pubmed|28830929]

reduced growth with ammonium as source of nitrogen due to the accumulation of toxic 5-oxoproline [pubmed|28830929]

The protein

Catalyzed reaction/ biological activity

5-oxoproline + ATP --> glutamate + ADP + Pi [pubmed|28830929]

The protein

Paralogous protein(s)

[[this]]

The protein

Kinetic information

Km (5-oxoproline): 39 M [pubmed|28830929]