Tue Dec 13 2016 11:16:29 GMT+0100 (CET)2025-05-11 07:01:27
description
general stress protein, survival of ethanol stress, SafA-dependent protein of the inner spore coat, spore cortex lytic protein
general stress protein, survival of ethanol stress, [[protein|SafA]]-dependent protein of the inner spore coat, spore cortex lytic protein
locus
BSU00160
BSU_00160
ec
outlinks
bsu
BSU00160
BSU_00160
aminos
MVKQGDTLSAIASQYRTTTNDITETNEIPNPDSLVVGQTIVIPIAGQFYDVKRGDTLTSIARQFNTTAAELARVNRIQLNTVLQIGFRLYIPPAPKRDIESNAYLEPRGNQVSENLQQAAREASPYLTYLGAFSFQAQRNGTLVAPPLTNLRSITESQNTTLMMIITNLENQAFSDELGRILLNDETVKRRLLNEIVENARRYGFRDIHFDFEYLRPQDREAYNQFLREARDLFHREGLEISTALAPKTSATQQGRWYEAHDYRAHGEIVDFVVLMTYEWGYSGGPPQAVSPIGPVRDVIEYALTEMPANKIVMGQNLYGYDWTLPYTAGGTPARAVSPQQAIVIADQNNASIQYDQTAQAPFFRYTDAENRRHEVWFEDARSIQAKFNLIKELNLRGISYWKLGLSFPQNWLLLSDQFNVVKKTFR
[SW|Categories] containing this gene/protein
[SW|sporulation proteins], [SW|general stress proteins (controlled by SigB)]
Gene
Coordinates on the chromosome (coding sequence)
23,868 -> 25,151
The protein
Paralogous protein(s)
[SW|YdhD]
[[this]]
The protein
[SW|Domains]
contains two N-acetylglucosamine-polymer-binding [SW|LysM domain]s at the N-terminus [Pubmed|18430080]
[SW|glycoside hydrolase family 18 domain ] (aa 125 to 410)
contains two N-acetylglucosamine-polymer-binding [SW|LysM domain]s at the N-terminus (aa 2-46, aa 51-95) [Pubmed|18430080]
[SW|glycoside hydrolase family 18 domain] (aa 125 to 410)
[SW|LysM domain] 1 (aa 2-46) (according to UniProt)
[SW|LysM domain] 2 (aa 51-95) (according to UniProt)
Expression and Regulation
Operon
''yaaH'' [Pubmed|10419957]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigE]] [Pubmed|15699190,10419957], [[protein|SigB]] [Pubmed|15805528]
Expression and Regulation
Regulation
induced by stress ([[protein|SigB]]) [Pubmed|15805528]
expressed during sporulation in the mother cell ([[protein|SigE]], [SW|SpoIIID]) [Pubmed|15383836,10419957]
Expression and Regulation
Regulatory mechanism
[SW|SpoIIID]: transcription repression [Pubmed|15383836]
Expression and Regulation
Additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
References
Original publications
proteinLength
427
geneLength
1284
Gene
Coordinates
23,868 25,151
The protein
Protein family
[SW|Glycosyl hydrolase 18 family] (according to UniProt)
Chitinase class II subfamily (with [[protein|YdhD]], according to UniProt)
The protein
Structure
[PDB|4S3K] (SleL from B. megaterium, 55% identity) [pubmed|26190134]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B889 (yaaH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1888 NBRP B. subtilis, Japan]
BKE00160 ([[gene|yaaH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATAAATTTGAATGAAAA, downstream forward: _UP4_TAATTTGGAAACGTCTTTTT
BKK00160 ([[gene|yaaH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATAAATTTGAATGAAAA, downstream forward: _UP4_TAATTTGGAAACGTCTTTTT