Tue Sep 20 2016 18:01:20 GMT+0200 (CEST)2025-05-22 12:55:12

locus

BSU25660

BSU_25660

outlinks

bsu

BSU25660

BSU_25660

[SW|Categories] containing this gene/protein

[SW|biosynthesis/ acquisition of amino acids]

Gene

Coordinates on the chromosome (coding sequence)

2,644,630 -> 2,645,472

The protein

Catalyzed reaction/ biological activity

Shikimate NADP = 3-dehydroshikimate NADPH (according to Swiss-Prot)

NADP⁺ + shikimate --> 3-dehydroshikimate + H⁺ + NADPH (according UniProt)

The protein

Protein family

shikimate dehydrogenase family (according to Swiss-Prot)

shikimate dehydrogenase family (single member, according to UniProt)

References

6811271, 24628944

6811271, 24628944, 22383849, 31358606

proteinLength

280

geneLength

843

Gene

Coordinates

2,644,630 2,645,472

Gene

Phenotypes of a mutant

slow growth [pubmed|31358606]

50% reduction in mo⁵U tRNA levels (because prephenate, the product formed by [[protein|AroH]], is used as hydroxyl donor for partial ho⁵U synthesis) [pubmed|31358606]

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE25660 ([[gene|aroD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25660 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_TTTCATACACCTCTCCCCTT, downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC

BKK25660 ([[gene|aroD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATACACCTCTCCCCTT, downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC