2020-04-17 16:30:352025-04-05 19:21:56
description
HNH nuclease-like protein, rescues AddA-AddB-mediated recombination intermediates
HNH nuclease-like protein, rescues [[protein|AddA]]-[[protein|AddB]]-mediated recombination intermediates
function
rescuing of AddA-AddB-mediated recombination intermediates
rescuing of [[protein|AddA]]-[[protein|AddB]]-mediated recombination intermediates
ec
Gene
Phenotypes of a mutant
essential, the mutation can be suppressed by second-site mutations in ''[[gene|addA]]'' or ''[[gene|addB]]'' [Pubmed|22984257], non-essential according to [Pubmed|28189581]
essential, the mutation can be suppressed by second-site mutations in [[gene|addA]] or [[gene|addB]] [Pubmed|22984257], non-essential according to [Pubmed|28189581]
Biological materials
Mutant
MGNA-A503 (yisB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/503 NBRP B. subtilis, Japan]
BKE10660 ([[gene|hlpB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTTGCCATATCCGCTC, downstream forward: _UP4_TGACTAAAAAGCAGCCCTCT
BKK10660 ([[gene|hlpB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTTGCCATATCCGCTC, downstream forward: _UP4_TGACTAAAAAGCAGCCCTCT
GP3559 (Δ[[gene|hlpB]]::kan), available in [SW|Jörg Stülke]'s lab
MGNA-A503 (yisB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/503 NBRP B. subtilis, Japan]
BKE10660 ([[gene|hlpB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTTGCCATATCCGCTC, downstream forward: _UP4_TGACTAAAAAGCAGCCCTCT
BKK10660 ([[gene|hlpB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTTGCCATATCCGCTC, downstream forward: _UP4_TGACTAAAAAGCAGCCCTCT
The protein
Paralogous protein(s)
[[this]]