Fri Jan 29 2016 17:48:45 GMT+0100 (CET)2025-04-30 11:17:07
locus
BSU24310
BSU_24310
essential
yes
no
outlinks
bsu
BSU24310
BSU_24310
[SW|Categories] containing this gene/protein
[SW|translation], [SW|essential genes]
Gene
Coordinates on the chromosome (coding sequence)
2,528,404 -> 2,529,255
Gene
Phenotypes of a mutant
essential [Pubmed|12682299]
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
inactivation of ''[[gene|folD]]'' suppresses the synthetic lethality of the ''[[gene|defA]] [[gene|defB]]'' double mutant [Pubmed|27983482]
The protein
Catalyzed reaction/ biological activity
5,10-methylenetetrahydrofolate
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP+ --> 5,10-methenyltetrahydrofolate + NADPH (according to UniProt)
5,10-methenyltetrahydrofolate + H2O --> (6S)-10-formyltetrahydrofolate + H+ (according to UniProt)
The protein
Protein family
tetrahydrofolate dehydrogenase/cyclohydrolase family (according to Swiss-Prot)
tetrahydrofolate dehydrogenase/cyclohydrolase family (single member, according to UniProt)
Expression and Regulation
Operon
''[[protein|nusB]]-[[protein|folD]]'' [Pubmed|2536750,11591660]
Expression and Regulation
Regulation
repressed in the presence of purine nucleotides ([[protein|PurR]]) [Pubmed|2536750,11591660]
Expression and Regulation
Regulatory mechanism
[[protein|PurR]]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750,11591660]
References
proteinLength
283
geneLength
852
Gene
Coordinates
2,528,404 2,529,255
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE24310 ([[gene|folD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCCTCCTATGA, downstream forward: _UP4_TAGATAATTGGAGATATTCA
BKK24310 ([[gene|folD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCCTCCTATGA, downstream forward: _UP4_TAGATAATTGGAGATATTCA