SubtiBank SubtiBank
Version comparison:

2017-02-27 15:02:042025-05-12 15:09:41

description

antagonist of biofilm repression by SinR, control of the phosphorelay

subunit of the regulatory iron-sulfur containing [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex, required for [[protein|Rny|RNase Y]] dependent maturation of polycistronic mRNAs, antagonist of biofilm repression by [[protein|SinR]], control of the [SW|phosphorelay]

locus

BSU14990

BSU_14990

geneLength

447

450

function

regulation of biofilm formation and the phosphorelay

control of [[protein|Rny|RNase Y]] activity, see [SW|Targets of the Y complex]

product

antagonist of biofilm repression by SinR, control of ComK stability

subunit of the regulatory iron-sulfur containing [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex

ec

names

ylbF

ylbF

ricF

outlinks

bsu

BSU14990

BSU_14990

Gene

Coordinates on the chromosome (coding sequence)

1,568,420 -> 1,568,869

Gene

Phenotypes of a mutant

strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]

strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]

defective in [[protein|Rny|RNase Y]]-mediated maturation of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]

The protein

[SW|Localization]

cytoplasm (according to Swiss-Prot)

cytoplasm [pubmed|27501195]

Expression and Regulation

[[this]]

References

10712692, 15661000, 23490197, 15175311, 26434553

Gene

Coordinates

1,568,420 1,568,869

The protein

Catalyzed reaction/ biological activity

the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex acts as specificity factor for [[protein|Rny|RNase Y]]-dependent processing of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]

the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex stimulates [[protein|Spo0A]] phosphorylation in the [[category|phosphorelay]] [pubmed|27501195]

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Cofactors]

one 4Fe-4S cluster (contributes to the co-ordination of the cluster (with [[protein|YmcA]] and [[protein|YaaT]])) [pubmed|31530674]

The protein

Structure

[PDB|6PRK] (the [[protein|YmcA]]-[[protein|YlbF]] complex) [pubmed|31530674]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Expression and Regulation

additional information

7,000/ 340 molecules per cell during growth in LB/ minimal medium [pubmed|31530674]

Biological materials

Mutant

BKE14990 ([[gene|ylbF]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14990 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG

BKK14990 ([[gene|ylbF]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG

References

Reviews

32061882, 32156829, 32156813

References

Original publications

10712692, 15661000, 23490197, 15175311, 26434553, 28295778, 27501195, 29794222, 31530674