2017-02-27 15:02:042025-05-12 15:09:41
description
antagonist of biofilm repression by SinR, control of the phosphorelay
subunit of the regulatory iron-sulfur containing [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex, required for [[protein|Rny|RNase Y]] dependent maturation of polycistronic mRNAs, antagonist of biofilm repression by [[protein|SinR]], control of the [SW|phosphorelay]
locus
BSU14990
BSU_14990
geneLength
447
450
function
regulation of biofilm formation and the phosphorelay
control of [[protein|Rny|RNase Y]] activity, see [SW|Targets of the Y complex]
product
antagonist of biofilm repression by SinR, control of ComK stability
subunit of the regulatory iron-sulfur containing [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex
ec
names
ylbF
ylbF
ricF
outlinks
bsu
BSU14990
BSU_14990
Gene
Coordinates on the chromosome (coding sequence)
1,568,420 -> 1,568,869
Gene
Phenotypes of a mutant
strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]
strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]
defective in [[protein|Rny|RNase Y]]-mediated maturation of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]
The protein
[SW|Localization]
cytoplasm (according to Swiss-Prot)
cytoplasm [pubmed|27501195]
Expression and Regulation
[[this]]
References
Gene
Coordinates
1,568,420 1,568,869
The protein
Catalyzed reaction/ biological activity
the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex acts as specificity factor for [[protein|Rny|RNase Y]]-dependent processing of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]
the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex stimulates [[protein|Spo0A]] phosphorylation in the [[category|phosphorelay]] [pubmed|27501195]
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Cofactors]
one 4Fe-4S cluster (contributes to the co-ordination of the cluster (with [[protein|YmcA]] and [[protein|YaaT]])) [pubmed|31530674]
The protein
Structure
[PDB|6PRK] (the [[protein|YmcA]]-[[protein|YlbF]] complex) [pubmed|31530674]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Expression and Regulation
additional information
7,000/ 340 molecules per cell during growth in LB/ minimal medium [pubmed|31530674]
Biological materials
Mutant
BKE14990 ([[gene|ylbF]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14990 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG
BKK14990 ([[gene|ylbF]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATGCACCTCCAGTT, downstream forward: _UP4_TGACGAGACTGGCCGTCTCG
References
Reviews
References
Original publications