Biological materials

Mutant

MGNA-B163 (yjbQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1162 NBRP B. subtilis, Japan]

GP2082 (''[[gene|cpaA]]''::''ermC''), available in [SW|Jörg Stülke]'s lab

BKE11640 ([[gene|cpaA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCAGAACCTCCTTTT, downstream forward: _UP4_TAAATCTTGACCAAATAAGG

BKK11640 ([[gene|cpaA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCAGAACCTCCTTTT, downstream forward: _UP4_TAAATCTTGACCAAATAAGG

MGNA-B163 (yjbQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1162 NBRP B. subtilis, Japan]

GP2082 (''[[gene|cpaA]]''::''ermC''), available in [SW|Jrg Stlke]'s lab

BKE11640 ([[gene|cpaA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCAGAACCTCCTTTT, downstream forward: _UP4_TAAATCTTGACCAAATAAGG

BKK11640 ([[gene|cpaA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCAGAACCTCCTTTT, downstream forward: _UP4_TAAATCTTGACCAAATAAGG

Biological materials

Expression vectors

pGP2909 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP2909 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jrg Stlke]'s lab

Biological materials

FLAG-tag construct

GP2438 ''yjbQ-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

GP2438 ''yjbQ-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jrg Stlke]'s lab