2017-8-11 16:0:32025-05-23 16:55:24
_ec
locus
BSU40740
BSU_40740
geneLength
519
522
outlinks
bsu
BSU40740
BSU_40740
Gene
Coordinates
4,185,160 → 4,185,681
4,185,160 4,185,681
The protein
Catalyzed reaction/ biological activity
acetylation of streptothricin at the expense of acetyl-CoA [pubmed|28842538]
acetylation of streptothricin at the expense of acetyl-CoA [pubmed|28842538]
acetyl-CoA + streptothricin D --> CoA + H+ + Nβ-acetylstreptothricin D (according to UniProt)
acetyl-CoA + streptothricin F --> CoA + H+ + Nβ-acetylstreptothricin F (according to UniProt)
The protein
Kinetic information
Km (streptothricine): 1 µM [pubmed|28842538]
Km (acetyl-CoA): 107 µM [pubmed|28842538]
Km (streptothricine): 1 M [pubmed|28842538]
Km (acetyl-CoA): 107 M [pubmed|28842538]
Biological materials
Mutant
MGNA-B856 (yyaR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1855 NBRP B. subtilis, Japan]
MGNA-B856 (yyaR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1855 NBRP B. subtilis, Japan]
BKE40740 ([[gene|satA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAATCTCCGCCAATC, downstream forward: _UP4_TAATTGATTATAAAACCTCA
BKK40740 ([[gene|satA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCAATCTCCGCCAATC, downstream forward: _UP4_TAATTGATTATAAAACCTCA
References
Research papers
The protein
Protein family
[SW|Acetyltransferase family] (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|N-acetyltransferase domain] (aa 21-173) (according to UniProt)