2017-12-14 12:30:572025-03-15 13:23:53

locus

BSU00110

BSU_00110

geneLength

882

885

outlinks

bsu

BSU00110

BSU_00110

Gene

Coordinates

19,062 → 19,946

19,062 19,946

Gene

Phenotypes of a mutant

auxotrophic for pyridoxal 5'-phosphate [Pubmed|14762015]

inactivation of ''[[gene|pdxS]]'' reduces sporulation efficiency to 1.2% that of wild type cells [Pubmed|26735940]

auxotrophic for pyridoxal 5'-phosphate [Pubmed|14762015]

inactivation of ''[[gene|pdxS]]'' reduces sporulation efficiency to 1.2% that of wild type cells [Pubmed|26735940]

poor growth [pubmed|28189581]

non-transformable [pubmed|28189581]

The protein

Protein family

pdxS/SNZ family (according to Swiss-Prot)

PdxS/SNZ family (single member, according to UniProt)

Biological materials

Mutant

BKE00110 (Δ[[gene|pdxS]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTGGTCCCCCTAAT, downstream forward: _UP4_TAAGAACATAGGAGCGCTGC

BKK00110 (Δ[[gene|pdxS]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTGGTCCCCCTAAT, downstream forward: _UP4_TAAGAACATAGGAGCGCTGC

BV604 (Δ([[gene|pdxS]]-[[gene|pdxT]])::tet), available in [SW|Fabian Commichau]'s lab, [Pubmed|24972371]

BKE00110 ([[gene|pdxS]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTGGTCCCCCTAAT, downstream forward: _UP4_TAAGAACATAGGAGCGCTGC

BKK00110 ([[gene|pdxS]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTGGTCCCCCTAAT, downstream forward: _UP4_TAAGAACATAGGAGCGCTGC

BV604 (([[gene|pdxS]]-[[gene|pdxT]])::tet), available in [SW|Fabian Commichau]'s lab, [Pubmed|24972371]

BP1100 (([[gene|pdxS]]-[[gene|pdxT]])::tet trp⁺), available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

References

15911615, 14762015, 25473090, 14651647, 17726680, 16493705, 16030023, 19152323, 17159152, 22517742, 15378759, 25568319, 26490441, 16233205

15911615, 14762015, 25473090, 14651647, 17726680, 16493705, 16030023, 19152323, 17159152, 22517742, 15378759, 25568319, 26490441, 16233205, 28189581

The protein

Catalyzed reaction/ biological activity

Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the [[protein|PdxT]] subunit. (according to UniProt)

aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine --> H⁺ + 3 H₂O + L-glutamate + phosphate + pyridoxal 5'-phosphate (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

Biological materials

Expression vector

pBP766: IPTG inducible expression, purification in E. coli with cleaveable N-terminal His-tag, in petSUMOadapt, available in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs

pBP772: expression of ''pdxST''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

pBP774: IPTG inducible expression and purification of ''pdxST'' in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

pBP775: constitutive expression of ''pdxS'' by [SW|pBQ200] in ''B. subtilis''