Tue Dec 13 2016 11:16:34 GMT+0100 (CET)2025-05-24 09:24:59
description
N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis (major enzyme)
N-acetylglucosamine-malate deacetylase, involved in bacillithiol synthesis (major enzyme)
locus
BSU22470
BSU_22470
product
N-acetylglucosamin-malate deacetylase
N-acetylglucosamine-malate deacetylase
ec
outlinks
bsu
BSU22470
BSU_22470
aminos
MYNADVLAFGAHSDDVEIGMGGTIAKFVKQEKKVMICDLTEAELSSNGTVSLRKEEAAEAARILGADKRIQLTLPDRGLIMSDQAIRSIVTVIRICRPKAVFMPYKKDRHPDHGNAAALVEEAIFSAGIHKYKDEKSLPAHKVSKVYYYMINGFHQPDFVIDISDTIEAKKQSLNAYKSQFIPSKDSVSTPLTNGYIEIVEAREKLYGKEAGVEYAEGFFSKRMLMLDHDVLGGEQ
[SW|Categories] containing this gene/protein
[SW|miscellaneous metabolic pathways], [SW|resistance against oxidative and electrophile stress]
Gene
Coordinates on the chromosome (coding sequence)
2,358,208 -> 2,358,918
The protein
Catalyzed reaction/ biological activity
GlcNAc-Mal -> GlcN-Mal + Ac
(S)-malyl N-acetyl-α-D-glucosaminide + H2O --> (S)-malyl α-D-glucosaminide + acetate (according to UniProt)
The protein
Paralogous protein(s)
[SW|BshB2]
[[this]]
Expression and Regulation
Operon
''[[protein|ypjD]]-[[protein|dapB]]-[[protein|mgsA]]-[SW|bshB1]-[[protein|bshA]]-[[protein|cca]]-[[protein|birA]]'' [Pubmed|20308541]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|23894131]
Expression and Regulation
Regulation
induced by diamide stess (thiol depletion) ([[protein|Spx]]) [Pubmed|23894131]
Expression and Regulation
Regulatory mechanism
[[protein|Spx]]: transcription activation [Pubmed|23894131]
Biological materials
Mutant
''bshB1'' null mutant and ''[SW|bshB1]'' / ''[SW|bshB2]'' double null mutant available in [SW|John Helmann] lab
GP87 (''ypjG''::''pX2''(''cat'')), available in [SW|Stülke] lab
MGNA-A409 (ypjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/409 NBRP B. subtilis, Japan]
''bshB1'' null mutant and ''[[gene|bshB1]]'' / ''[[gene|bshB2]]'' double null mutant available in [SW|John Helmann] lab
GP87 (''ypjG''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab
BKE22470 ([[gene|bshB1]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG
BKK22470 ([[gene|bshB1]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG
References
proteinLength
236
geneLength
711
Gene
Coordinates
2,358,208 2,358,918
The protein
Protein family
PIGL family (with [[protein|BshB2]], according to UniProt)
The protein
[SW|Cofactors]
Zn2+ [pubmed|31867856]
The protein
Structure
[PDB|6ULL] (complex with substrate analogue) [pubmed|31867856]
[PDB|6P2T] (complex with citrate) [pubmed|31867856]
[PDB|2IXD] (from B. cereus, 62% identity) [pubmed|17501983]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
References
Reviews
References
Original Publications