Mon Aug 31 2015 13:55:30 GMT+0200 (CEST)2025-06-01 12:44:59
description
putative methyl-cis-aconitase
2-methylcitrate dehydratase
locus
BSU24130
BSU_24130
product
unknown
2-methylcitrate dehydratase
outlinks
bsu
BSU24130
BSU_24130
syns
yqiP, prpD
[SW|Categories] containing this gene/protein
[SW|sporulation proteins], [SW|poorly characterized/ putative enzymes]
The protein
Catalyzed reaction/ biological activity
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O (according to Swiss-Prot)
2-methylcitrate --> 2-methylaconitate [pubmed|28956599]
citrate --> cis-aconitate + H2O (according to UniProt)
The protein
Protein family
prpD family (according to Swiss-Prot)
prpD family (with [[protein|YxeQ]], according to UniProt)
The protein
[SW|Cofactors]
contains an iron-sulfur cluster
Fe-S cluster [pubmed|29292548]
Expression and Regulation
Operon
''[[protein|mmgA]]-[[protein|mmgB]]-[[protein|mmgC]]-[[protein|mmgD]]-[[protein|mmgE]]-[[protein|yqiQ]]'' [Pubmed|8759838]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigE]] [Pubmed|15699190,8759838]
Expression and Regulation
Regulation
expressed early during sporulation in the mother cell ([[protein|SigE]]) [Pubmed|15699190,8759838]
repressed by glucose ([[protein|CcpA]]) [Pubmed|8759838]
Expression and Regulation
Regulatory mechanism
[[protein|CcpA]]: transcription repression [Pubmed|8759838]
References
proteinLength
472
geneLength
1419
Gene
Coordinates
2,508,221 2,509,639
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|5MUX]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE24130 ([[gene|mmgE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCGGCATTCTACCAGCTC, downstream forward: _UP4_ATGTAAAAGGGGGATTTTGT
BKK24130 ([[gene|mmgE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCGGCATTCTACCAGCTC, downstream forward: _UP4_ATGTAAAAGGGGGATTTTGT