Mon Aug 31 2015 13:55:25 GMT+0200 (CEST)2025-05-16 10:57:03
locus
BSU23570
BSU_23570
outlinks
bsu
BSU23570
BSU_23570
syns
[SW|Categories] containing this gene/protein
[SW|utilization of amino acids], [SW|membrane proteins]
The protein
Catalyzed reaction/ biological activity
L-aspartate = fumarate + NH3 (according to Swiss-Prot)
L-aspartate --> fumarate + NH4+ (according to UniProt)
The protein
Protein family
Aspartase subfamily (according to Swiss-Prot)
class-II fumarase/aspartase family (with [[protein|CitG]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|CitG]]
[[this]]
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operon
''[[protein|ansA]]-[[protein|ansB]]'' [Pubmed|1711029]
''[[protein|ansA]]-[[protein|ansB]]-[[protein|mleN]]-[[protein|mleA]]'' [Pubmed|22383849]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|1711029]
Expression and Regulation
Regulation
expressed in the presence of asparagine ([[protein|AnsR]]) [Pubmed|11914346]
Expression and Regulation
Regulatory mechanism
[[protein|AnsR]]: transcription repression [Pubmed|11914346]
Biological materials
Mutant
GP1153 (del ''ansAB''::''ermC'') available in the [SW|Stülke] lab
GP1153 (Δ[[gene|ansA]]-[[gene|ansB]]::''ermC'') available in [SW|Jörg Stülke]'s lab
BKE23570 ([[gene|ansB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTAACCTTCTTT, downstream forward: _UP4_TAAAATTTGTATAAAATACA
BKK23570 ([[gene|ansB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTAACCTTCTTT, downstream forward: _UP4_TAAAATTTGTATAAAATACA
proteinLength
475
geneLength
1428
Gene
Coordinates
2,454,347 2,455,774
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
lacZ fusion
pGP2274 (in [SW|pAC5]) (GP2961), available in [SW|Jörg Stülke]'s lab