SubtiBank SubtiBank
Version comparison:

Tue Dec 13 2016 11:16:36 GMT+0100 (CET)2025-05-09 06:47:12

locus

BSU34030

BSU_34030

ec

outlinks

bsu

BSU34030

BSU_34030

aminos

MTKGTIQNQESFLNRIASSLGRERRTGGVAVPEWAHQPQYKTLEGYSADDLVTVLKNHCVKIHTELIETDSTGLYDALREQVSRFSGGPVIIPKDPRFEEYGLKSLLTKDWPSEGTPVWEWDADKGEENIKKAEQANVGITFSEITLAESGTVVLFSSKDIGRSVSLLPTTYIAIVPKSSIVPRMTQASDIIRQNIANGVTVPSCINYITGPSNSADIEMDLVVGVHGPVKAAYILVSDR

[SW|Categories] containing this gene/protein

[SW|utilization of specific carbon sources], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence)

3,492,797 -> 3,493,519

The protein

Protein family

UPF0707 family (according to Swiss-Prot)

LutC/YkgG family (single member, according to UniProt)

Expression and Regulation

Operon

''[[protein|lutA]]-[[protein|lutB]]-[[protein|lutC]]'' [Pubmed|19201793]

Expression and Regulation

Regulation

induction by lactate [Pubmed|19201793]

repressed by [[protein|SinR]] [Pubmed|19201793]

part of the iron sparing response, down-regulation in a'' [[protein|fur]]'' mutant ([[protein|Fur]], [[protein|FsrA]], [[protein|FpbB]]) [Pubmed|22427629]

Expression and Regulation

Regulatory mechanism

dual repression by [[protein|LutR]] (inducer lactate) and [[protein|SinR]] [Pubmed|19201793]

[[protein|SinR]]: transcription repression [Pubmed|19201793]

[[protein|FsrA]]: translational repression [Pubmed|22427629]

[[protein|FbpB]]: acts as RNA chaperone, increases interaction between ''[[protein|fsrA]]'' and ''[[protein|lutA]]-[[protein|lutB]]-[[protein|lutC]]'' RNAs [Pubmed|22427629]

Labs working on this gene/protein

[SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

proteinLength

240

geneLength

723

Gene

Coordinates

3,492,797 3,493,519

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-A470 (yvbY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/470 NBRP B. subtilis, Japan]

BKE34030 ([[gene|lutC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTCTGAATGGTTCCCTTCG, downstream forward: _UP4_TGAACTCAGGAAGCCCGGCA

BKK34030 ([[gene|lutC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTCTGAATGGTTCCCTTCG, downstream forward: _UP4_TGAACTCAGGAAGCCCGGCA

labs

[SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]