2018-04-29 08:33:032025-05-27 21:34:34
description
similar to rRNA adenosine methyltransferase for modification of 23S rRNA
similar to RNA adenosine methyltransferase
locus
BSU15750
BSU_15750
geneLength
1089
1092
function
rRNA modification
RNA modification
product
rRNA methyltransferase
RNA methyltransferase
outlinks
bsu
BSU15750
BSU_15750
Gene
Coordinates
1,649,286 → 1,650,377
1,649,286 1,650,377
The protein
Protein family
RlmN family (according to Swiss-Prot), SAM enzyme superfamily
[SW|Radical SAM superfamily] (according to UniProt)
Biological materials
Mutant
MGNA-B132 (yloN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1131 NBRP B. subtilis, Japan]
1A819 ( ''yloN''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A819&Search=1A819 BGSC]
BKE15750 (Δ[[gene|yloN]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTTTATTAAGTTCTGCCA, downstream forward: _UP4_CAAGACGAGACGAGGTGATG
BKK15750 (Δ[[gene|yloN]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTTTATTAAGTTCTGCCA, downstream forward: _UP4_CAAGACGAGACGAGGTGATG
MGNA-B132 (yloN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1131 NBRP B. subtilis, Japan]
1A819 ( ''yloN''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A819&Search=1A819 BGSC]
BKE15750 ([[gene|yloN]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTTTATTAAGTTCTGCCA, downstream forward: _UP4_CAAGACGAGACGAGGTGATG
BKK15750 ([[gene|yloN]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTTTATTAAGTTCTGCCA, downstream forward: _UP4_CAAGACGAGACGAGGTGATG
References
The protein
Catalyzed reaction/ biological activity
methylates C-2 in A2503 in 23S rRNA and A37 in tRNA [pubmed|23752511]
adenosine2503 in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2-methyladenosine2503 in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine (according to UniProt)
adenosine37 in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2-methyladenosine37 in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|3RF9] (from E. coli, 38% identity) [pubmed|21527678]