Tue Dec 13 2016 11:16:36 GMT+0100 (CET)2025-05-31 22:35:33
description
UDP-sugar epimerase, required for extracellular polysaccharide synthesis, this gene is inactive in B. subtilis 168
UDP-N-acetylglucosamine 4,6-dehydratase, required for extracellular polysaccharide synthesis, this gene is inactive in B. subtilis 168
locus
BSU34350
BSU_34350
function
biofilm formation
[[category|Biofilm formation]], biosynthesis of N,N'-diacetylbacillosamine
product
unknown
UDP-N-acetylglucosamine 4,6-dehydratase
ec
outlinks
bsu
BSU34350
BSU_34350
aminos
MIIALDTYLVLNSVIAGYQFLKDSYQFYDSGALLLTAVSLLLSYHVCAFLFNQYKQVWTYTGLGELIVLLKGITLSAAVTGVIQYAVYHTMFFRLLTACWVLQLLSIGGTRILSRVLNESIRKKRCASSRALIIGAGSGGTLMVRQLLSKDEPDIIPVAFIDDDQTKHKLEIMGLPVIGGKESIMPAVQKLKINYIIIAIPSLRTHELQVLYKECVRTGVSIKIMPHFDEMLLGTRTAGQIRDVKAEDLLGRKPVTLDTSEISNRIKGKTVLVTGAGGSIGSEICRQISAFQPKEIILLGHGENSIHSIYTELNGRFGKHIVFHTEIADVQDRDKMFTLMKKYEPHVVYHAAAHKHVPLMEHNPEEAVKNNIIGTKNVAEAADMSGTETFVLISSDKAVNPANVMGATKRFAEMIIMNLGKVSRTKFVAVRFGNVLGSRGSVIPIFKKQIEKGGPVTVTHPAMTRYFMTIPEASRLVIQAGALAKGRQIFVLDMGEPVKIVDLAKNLIHLSGYTTEQVPIEFTGIRPGEKMYEELLNKNEVHAEQIFPKIHIGKAVDGDWPVLMRFIEDFHELPEADLRARLFAAINTSEEMTAASVH
[SW|Categories] containing this gene/protein
[SW|biofilm formation], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
3,526,407 -> 3,528,203
The protein
Protein family
polysaccharide synthase family (according to Swiss-Prot)
[SW|Polysaccharide synthase family] (according to UniProt)
The protein
Paralogous protein(s)
SpsM ([[protein|YodU]], [[protein|YpqP]])
[[this]]
Expression and Regulation
Operon
''[[protein|epsA]]-[[protein|epsB]]-[[protein|epsC]]-[[protein|epsD]]-[[protein|epsE]]-[[protein|epsF]]-[[protein|epsG]]-[[protein|epsH]]-[[protein|epsI]]-[[protein|epsJ]]-[[protein|epsK]]-[[protein|epsL]]-[[protein|epsM]]-[[protein|epsN]]-[[protein|epsO]]'' [Pubmed|15661000]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|15661000]
Expression and Regulation
Regulation
repressed by [[protein|SinR]] [Pubmed|15661000]
Expression and Regulation
Regulatory mechanism
[[protein|SinR]]: transcription anti-activation (prevents binding of [[protein|RemA]]) [Pubmed|23646920]
[[protein|RemA]]: transcription activation [Pubmed|23646920]
[[protein|AbrB]]: transcription repression [Pubmed|20817675]
Expression and Regulation
Additional information
induction by sequestration of [[protein|SinR]] by [[protein|SinI]] or [[protein|SlrA]] [PubMed|15661000,19788541]
the [[EAR riboswitch]] (eps-associated [[RNA switch]]) located between' [[protein|epsB]]' and '[[protein|epsC]]' mediates processive antitermination and allows expression of the long eps operon [PubMed|20374491]
the '[[protein|epsA]]-[[protein|epsB]]-[[protein|epsC]]-[[protein|epsD]]-[[protein|epsE]]-[[protein|epsF]]-[[protein|epsG]]-[[protein|epsH]]-[[protein|epsI]]-[[protein|epsJ]]-[[protein|epsK]]-[[protein|epsL]]-[[protein|epsM]]-[[protein|epsN]]-[[protein|epsO]]' operon is not expressed in a '[[protein|ymdB]]' mutant [PubMed|21856853]
the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] (this is likely due to the increased stability of the '[[protein|sinR]]' mRNA) [PubMed|21815947]
=The EAR [SW|RNA switch]=
proteinLength
598
geneLength
1797
Gene
Coordinates
3,526,407 3,528,203
The protein
[SW|Cofactors]
NAD+ [pubmed|30222950]
The protein
Structure
[PDB|5BJW] (from Campylobacter jejuni, corresponds to the C-terminal part of EpsC, aa 242 ... 571, 47% identity) [pubmed|29053280]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A071 (yveM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/71 NBRP B. subtilis, Japan]
BKE34350 ([[gene|epsC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGACAGTCTTCTCCGGT, downstream forward: _UP4_AGCGTTCATTAGGGGGAGTG
BKK34350 ([[gene|epsC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGACAGTCTTCTCCGGT, downstream forward: _UP4_AGCGTTCATTAGGGGGAGTG
References
Research papers
References
The EAR [SW|RNA switch]