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Tue Dec 13 2016 11:16:36 GMT+0100 (CET)2025-05-31 22:35:33

description

UDP-sugar epimerase, required for extracellular polysaccharide synthesis, this gene is inactive in B. subtilis 168

UDP-N-acetylglucosamine 4,6-dehydratase, required for extracellular polysaccharide synthesis, this gene is inactive in B. subtilis 168

locus

BSU34350

BSU_34350

function

biofilm formation

[[category|Biofilm formation]], biosynthesis of N,N'-diacetylbacillosamine

product

unknown

UDP-N-acetylglucosamine 4,6-dehydratase

ec

outlinks

bsu

BSU34350

BSU_34350

aminos

MIIALDTYLVLNSVIAGYQFLKDSYQFYDSGALLLTAVSLLLSYHVCAFLFNQYKQVWTYTGLGELIVLLKGITLSAAVTGVIQYAVYHTMFFRLLTACWVLQLLSIGGTRILSRVLNESIRKKRCASSRALIIGAGSGGTLMVRQLLSKDEPDIIPVAFIDDDQTKHKLEIMGLPVIGGKESIMPAVQKLKINYIIIAIPSLRTHELQVLYKECVRTGVSIKIMPHFDEMLLGTRTAGQIRDVKAEDLLGRKPVTLDTSEISNRIKGKTVLVTGAGGSIGSEICRQISAFQPKEIILLGHGENSIHSIYTELNGRFGKHIVFHTEIADVQDRDKMFTLMKKYEPHVVYHAAAHKHVPLMEHNPEEAVKNNIIGTKNVAEAADMSGTETFVLISSDKAVNPANVMGATKRFAEMIIMNLGKVSRTKFVAVRFGNVLGSRGSVIPIFKKQIEKGGPVTVTHPAMTRYFMTIPEASRLVIQAGALAKGRQIFVLDMGEPVKIVDLAKNLIHLSGYTTEQVPIEFTGIRPGEKMYEELLNKNEVHAEQIFPKIHIGKAVDGDWPVLMRFIEDFHELPEADLRARLFAAINTSEEMTAASVH

[SW|Categories] containing this gene/protein

[SW|biofilm formation], [SW|membrane proteins]

Gene

Coordinates on the chromosome (coding sequence)

3,526,407 -> 3,528,203

The protein

Protein family

polysaccharide synthase family (according to Swiss-Prot)

[SW|Polysaccharide synthase family] (according to UniProt)

The protein

Paralogous protein(s)

SpsM ([[protein|YodU]], [[protein|YpqP]])

[[this]]

Expression and Regulation

Operon

''[[protein|epsA]]-[[protein|epsB]]-[[protein|epsC]]-[[protein|epsD]]-[[protein|epsE]]-[[protein|epsF]]-[[protein|epsG]]-[[protein|epsH]]-[[protein|epsI]]-[[protein|epsJ]]-[[protein|epsK]]-[[protein|epsL]]-[[protein|epsM]]-[[protein|epsN]]-[[protein|epsO]]'' [Pubmed|15661000]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|15661000]

Expression and Regulation

Regulation

repressed by [[protein|SinR]] [Pubmed|15661000]

Expression and Regulation

Regulatory mechanism

[[protein|SinR]]: transcription anti-activation (prevents binding of [[protein|RemA]]) [Pubmed|23646920]

[[protein|RemA]]: transcription activation [Pubmed|23646920]

[[protein|AbrB]]: transcription repression [Pubmed|20817675]

Expression and Regulation

Additional information

induction by sequestration of [[protein|SinR]] by [[protein|SinI]] or [[protein|SlrA]] [PubMed|15661000,19788541]

the [[EAR riboswitch]] (eps-associated [[RNA switch]]) located between' [[protein|epsB]]' and '[[protein|epsC]]' mediates processive antitermination and allows expression of the long eps operon [PubMed|20374491]

the '[[protein|epsA]]-[[protein|epsB]]-[[protein|epsC]]-[[protein|epsD]]-[[protein|epsE]]-[[protein|epsF]]-[[protein|epsG]]-[[protein|epsH]]-[[protein|epsI]]-[[protein|epsJ]]-[[protein|epsK]]-[[protein|epsL]]-[[protein|epsM]]-[[protein|epsN]]-[[protein|epsO]]' operon is not expressed in a '[[protein|ymdB]]' mutant [PubMed|21856853]

the amount of the mRNA is substantially decreased upon depletion of [[Rny|RNase Y]] (this is likely due to the increased stability of the '[[protein|sinR]]' mRNA) [PubMed|21815947]

=The EAR [SW|RNA switch]=

20374491, 20230605

proteinLength

598

geneLength

1797

Gene

Coordinates

3,526,407 3,528,203

The protein

[SW|Cofactors]

NAD+ [pubmed|30222950]

The protein

Structure

[PDB|5BJW] (from Campylobacter jejuni, corresponds to the C-terminal part of EpsC, aa 242 ... 571, 47% identity) [pubmed|29053280]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-A071 (yveM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/71 NBRP B. subtilis, Japan]

BKE34350 ([[gene|epsC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGACAGTCTTCTCCGGT, downstream forward: _UP4_AGCGTTCATTAGGGGGAGTG

BKK34350 ([[gene|epsC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGACAGTCTTCTCCGGT, downstream forward: _UP4_AGCGTTCATTAGGGGGAGTG

References

Research papers

27655338, 30222950, 29053280

References

The EAR [SW|RNA switch]

20374491, 20230605