SubtiBank SubtiBank
Version comparison:

Fri Jun 10 2016 15:33:46 GMT+0200 (CEST)2025-05-24 06:44:49

locus

BSU00280

BSU_00280

outlinks

bsu

BSU00280

BSU_00280

[SW|Categories] containing this gene/protein

[SW|biosynthesis/ acquisition of nucleotides], [SW|essential genes]

Gene

Coordinates on the chromosome (coding sequence)

39,159 -> 39,797

The protein

Catalyzed reaction/ biological activity

ATP dTMP = ADP dTDP (according to Swiss-Prot)

ATP + dTMP --> ADP + dTDP (according to UniProt)

The protein

Protein family

thymidylate kinase family (according to Swiss-Prot)

Thymidylate kinase family (single member, according to UniProt)

Biological materials

Expression vector

pGP1689: expression of Strep-''tmk'' in ''E. coli'', in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

pGP2671: expression of ''tmk''-Strep, in [SW|pGP380] ,in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

Biological materials

FLAG-tag construct

GP2401 ''tmk-3xFLAG spc'' (based on [SW|pGP2786]), available in [SW|Jörg Stülke]'s lab

GP2401 ''tmk-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

16522804, 18523102, 23043329

16522804, 18523102, 23043329, 18523102, 27376462, 23394555

proteinLength

212

geneLength

639

Gene

Coordinates

39,159 39,797

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Localization]

cytoplasm (according to UniProt)

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

pGP1689: expression of Strep-''tmk'' in ''E. coli'', in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

pGP2671: expression of ''tmk''-Strep, in [SW|pGP380] ,in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab