Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-14 12:55:55
description
Spx-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein
[[protein|Spx]]-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein
locus
BSU38110
BSU_38110
ec
outlinks
bsu
BSU38110
BSU_38110
aminos
MNNTIETILNHRSIRSFTDQLLTAEEIDTLVKSAQAASTSSYVQAYSIIGVSDPEKKRELSVLAGNQPYVEKNGHFFVFCADLYRHQQLAEEKGEHISELLENTEMFMVSLIDAALAAQNMSIAAESMGLGICYIGGIRNELDKVTEVLQTPDHVLPLFGLAVGHPANLSGKKPRLPKQAVYHENTYNVNTDDFRHTMNTYDKTISDYYRERTNGKREETWSDQILNFMKQKPRTYLNDYVKEKGFNKN
[SW|Categories] containing this gene/protein
[SW|electron transport/ other], [SW|resistance against oxidative and electrophile stress]
Gene
Coordinates on the chromosome (coding sequence)
3,911,477 -> 3,912,226
The protein
Catalyzed reaction/ biological activity
possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD( ) degrading activity leading to free nicotinamide [Pubmed|20727352]
possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD( ) degrading activity leading to free nicotinamide [Pubmed|20727352]
FMNH2 + NADP+ --> FMN + 2 H+ + NADPH (according to UniProt)
The protein
Protein family
flavin oxidoreductase frp family (according to Swiss-Prot)
flavin oxidoreductase frp family (with [[protein|YcnD]], according to UniProt)
Expression and Regulation
Operon
''[[protein|nfrA]]-[[protein|ywcH]]'' [Pubmed|10913069]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|10913069], [[protein|SigD]] [Pubmed|9535080]
Expression and Regulation
Regulation
repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
Expression and Regulation
Regulatory mechanism
[SW|Spo0A]: transcription repression [Pubmed|14651647]
[[protein|Spx]]: transcription activation [Pubmed|12642660,19575568]
References
proteinLength
249
geneLength
750
Gene
Coordinates
3,911,477 3,912,226
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-B232 (ywcG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1231 NBRP B. subtilis, Japan]
BKE38110 ([[gene|nfrA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACAAAAACCTCCTTAT, downstream forward: _UP4_TAAAGCAAACCTCTCCGTCT
BKK38110 ([[gene|nfrA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACAAAAACCTCCTTAT, downstream forward: _UP4_TAAAGCAAACCTCTCCGTCT