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Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-14 12:55:55

description

Spx-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein

[[protein|Spx]]-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein

locus

BSU38110

BSU_38110

ec

outlinks

bsu

BSU38110

BSU_38110

aminos

MNNTIETILNHRSIRSFTDQLLTAEEIDTLVKSAQAASTSSYVQAYSIIGVSDPEKKRELSVLAGNQPYVEKNGHFFVFCADLYRHQQLAEEKGEHISELLENTEMFMVSLIDAALAAQNMSIAAESMGLGICYIGGIRNELDKVTEVLQTPDHVLPLFGLAVGHPANLSGKKPRLPKQAVYHENTYNVNTDDFRHTMNTYDKTISDYYRERTNGKREETWSDQILNFMKQKPRTYLNDYVKEKGFNKN

[SW|Categories] containing this gene/protein

[SW|electron transport/ other], [SW|resistance against oxidative and electrophile stress]

Gene

Coordinates on the chromosome (coding sequence)

3,911,477 -> 3,912,226

The protein

Catalyzed reaction/ biological activity

possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD( ) degrading activity leading to free nicotinamide [Pubmed|20727352]

possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD( ) degrading activity leading to free nicotinamide [Pubmed|20727352]

FMNH2 + NADP+ --> FMN + 2 H+ + NADPH (according to UniProt)

The protein

Protein family

flavin oxidoreductase frp family (according to Swiss-Prot)

flavin oxidoreductase frp family (with [[protein|YcnD]], according to UniProt)

Expression and Regulation

Operon

''[[protein|nfrA]]-[[protein|ywcH]]'' [Pubmed|10913069]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|10913069], [[protein|SigD]] [Pubmed|9535080]

Expression and Regulation

Regulation

repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Expression and Regulation

Regulatory mechanism

[SW|Spo0A]: transcription repression [Pubmed|14651647]

[[protein|Spx]]: transcription activation [Pubmed|12642660,19575568]

References

9535080, 16847875, 10913069, 12642660, 14651647, 20727352, 9836433, 24847778

9535080, 16847875, 10913069, 12642660, 14651647, 20727352, 9836433, 24847778, 29271514

proteinLength

249

geneLength

750

Gene

Coordinates

3,911,477 3,912,226

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-B232 (ywcG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1231 NBRP B. subtilis, Japan]

BKE38110 ([[gene|nfrA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACAAAAACCTCCTTAT, downstream forward: _UP4_TAAAGCAAACCTCTCCGTCT

BKK38110 ([[gene|nfrA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACAAAAACCTCCTTAT, downstream forward: _UP4_TAAAGCAAACCTCTCCGTCT