Wed Mar 09 2016 16:15:47 GMT+0100 (CET)2025-05-30 03:19:53
description
transcriptional regulator (Xre family) of post-exponential-phase responses genes
transcriptional regulator ([SW|Xre family]) of post-exponential-phase responses genes
locus
BSU24610
BSU_24610
function
control of biofilm formation
control of [SW|biofilm formation]
product
transcriptional regulator (Xre family)
transcriptional regulator ([SW|Xre family])
ec
outlinks
bsu
BSU24610
BSU_24610
[SW|Categories] containing this gene/protein
[SW|transcription factors and their control], [SW|transition state regulators], [SW|biofilm formation]
Gene
Coordinates on the chromosome (coding sequence)
2,552,653 -> 2,552,988
Gene
Phenotypes of a mutant
the mutation suppresses the galactose toxicity to a'' [[protein|galE]]'' mutant [Pubmed|22893383]
the mutation suppresses the galactose toxicity to a'' [[gene|galE]]'' mutant [Pubmed|22893383]
The protein
Catalyzed reaction/ biological activity
transcription regulator of biofilm genes, acts as a true repressor of the ''[[protein|tapA]]-[[protein|sipW]]-[[protein|tasA]]'' operon and as an anti-activator (prevents binding of the activator protein [[protein|RemA]]) of the ''[[protein|epsA]]-[[protein|epsB]]-[[protein|epsC]]-[[protein|epsD]]-[[protein|epsE]]-[[protein|epsF]]-[[protein|epsG]]-[[protein|epsH]]-[[protein|epsI]]-[[protein|epsJ]]-[[protein|epsK]]-[[protein|epsL]]-[[protein|epsM]]-[[protein|epsN]]-[[protein|epsO]]'' operon [Pubmed|23646920]
acts as co-repressor for [[protein|SlrR]] [Pubmed|20351052]
transcription regulator of biofilm genes, acts as a true repressor of the ''[[gene|tapA]]-[[gene|sipW]]-[[gene|tasA]]'' operon and as an anti-activator (prevents binding of the activator protein [[protein|RemA]]) of the ''[[gene|epsA]]-[[gene|epsB]]-[[gene|epsC]]-[[gene|epsD]]-[[gene|epsE]]-[[gene|epsF]]-[[gene|epsG]]-[[gene|epsH]]-[[gene|epsI]]-[[gene|epsJ]]-[[gene|epsK]]-[[gene|epsL]]-[[gene|epsM]]-[[gene|epsN]]-[[gene|epsO]]'' operon [Pubmed|23646920]
acts as co-repressor for [[protein|SlrR]] [Pubmed|20351052]
The protein
Paralogous protein(s)
[[protein|SlrR]]
[[this]]
The protein
[SW|Domains]
DNA-binding N-terminal domain (aa 1-69) [Pubmed|21708175]
[[protein|SinI]]-binding C-terminal domain (aa 74-111) [Pubmed|21708175]
DNA-binding N-terminal domain (aa 1-69) [Pubmed|21708175]
[[protein|SinI]]-binding C-terminal domain (aa 74-111) [Pubmed|21708175]
[SW|HTH cro/C1-type domain] (aa 6-61) (according to UniProt)
[SW|Sin domain] (aa 65-103) (according to UniProt)
The protein
[SW|Interactions]
[[protein|SlrA]]-[[protein|SinR]] [Pubmed|19788541], K(D) 10.6 nM [Pubmed|23430750]
[[protein|SlrR]]-[[protein|SinR]] [Pubmed|20351052], K(D) 47.5 nM [Pubmed|23430750], [[protein|Veg]] may inhibit this interaction [Pubmed|23378512]
[[protein|SinR]]-[[protein|SinI]] [Pubmed|9799632], K(D) 1.8 nM [Pubmed|23430750]
[[protein|SinR]]-[[protein|ScoC]]
Expression and Regulation
Operon
''[[protein|sinI]]-[[protein|sinR]]'' [Pubmed|3125149]
''[[protein|sinR]]'' [Pubmed|3125149]
Expression and Regulation
[SW|Sigma factor]
''[[protein|sinI]]'': [[protein|SigA]] [Pubmed|3125149]
''[[protein|sinR]]'': [[protein|SigA]] [Pubmed|3125149]
Expression and Regulation
Regulation
''[[protein|sinI]]'': repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|11751836]
''[[protein|sinI]]'': repressed during exponential growth ([[protein|ScoC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
''[[protein|sinI]]'': repressed during logrithmic growth ([[protein|AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7635837,11751836 PubMed]
''[[protein|sinI]]'': repressed during vegetative growth ([[protein|SinR]]) [Pubmed|1664536]
''[[protein|sinR]]'' is bimodally expressed and this depends on [[rny|RNase Y]] [Pubmed|26819068]
Expression and Regulation
Regulatory mechanism
[SW|Spo0A]: transcription repression [Pubmed|11751836]
[[protein|ScoC]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
[[protein|AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/7635837,11751836 PubMed]
[[protein|SinR]]: transcription repression [Pubmed|1664536]
Expression and Regulation
Additional information
the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] (the half-life of the mRNA increases from 3.5 to 13 min) [PubMed|21815947]
Biological materials
Mutant
GP923 (''sinR::spec'') [Pubmed|21856853], available in [SW|Jörg Stülke]'s lab
GP736 (''sinR::tetR'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab
1S97 (''sinR''::''phleo''), [Pubmed|8422983], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S97&Search=1S97 BGSC]
GP1672 (''[[protein|sinR]]-[[protein|tasA]]''::''cat'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab
GP1663 (''[[protein|yqhG]]-[[protein|sinI]]-[[protein|sinR]]-[[protein|tasA]]''), available in [SW|Jörg Stülke]'s lab
GP923 (''sinR::spec'') [Pubmed|21856853], available in [SW|Jörg Stülke]'s lab
GP736 (''sinR::tetR'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab
1S97 (''sinR''::''phleo''), [Pubmed|8422983], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S97&Search=1S97 BGSC]
GP1672 (''[[gene|sinR]]-[[gene|tasA]]''::''cat'') [Pubmed|24493247], available in [SW|Jörg Stülke]'s lab
GP1663 (''[[gene|yqhG]]-[[gene|sinI]]-[[gene|sinR]]-[[gene|tasA]]''), available in [SW|Jörg Stülke]'s lab
BKE24610 ([[gene|sinR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTCATCACCTTCCTTG, downstream forward: _UP4_TAGTGCCTGAGCAGAGGCAC
BKK24610 ([[gene|sinR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTCATCACCTTCCTTG, downstream forward: _UP4_TAGTGCCTGAGCAGAGGCAC
Biological materials
Expression vector
N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]: pGP1083 , available in [SW|Jörg Stülke]'s lab
Biological materials
lacZ fusion
pGP1930 (aphA3) based on [[protein|pAC7]], available in [SW|Jörg Stülke]'s lab
pGP1930 (aphA3) based on [SW|pAC7], available in [SW|Jörg Stülke]'s lab
References
Reviews
References
Original publications
proteinLength
111
geneLength
336
Gene
Coordinates
2,552,653 2,552,988
The protein
additional information
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000086 PRODORIC2 database]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]: pGP1083 , available in [SW|Jörg Stülke]'s lab
pGP2330: in [SW|pBQ200], for expression in B. subtilis, available in [SW|Jörg Stülke]'s lab