Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-16 08:14:43
title
ydaO
kimA
description
putative transporter
high-affinity potassium transporter (uptake)
locus
BSU04320
BSU_04320
function
unknown
potassium uptake
product
unknown
high-affinity K+/H+ symporter
ec
names
ydaO
kimA
ydaO
outlinks
bsu
BSU04320
BSU_04320
[SW|Categories] containing this gene/protein
[SW|membrane proteins], [SW|proteins of unknown function]
Gene
Coordinates on the chromosome (coding sequence)
486,432 -> 488,255
The protein
[SW|Localization]
cell membrane (according to [http://www.uniprot.org/uniprot/P96589 UniProt])
cell membrane [pubmed|30452022]
Expression and Regulation
Operon
''[[protein|ydaO]]'' [Pubmed|22383849]
Expression and Regulation
Regulatory mechanism
expression is controlled via termination antitermination by the [SW|ydaO riboswitch], expression is switched off upon binding od c-di-AMP [Pubmed|24141192,20511502]
Biological materials
Mutant
1A688 (''[[protein|ydaO]]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]
GP93 (''[[protein|ydaO]]''::''cat''), available in [SW|Jörg Stülke]'s lab
MGNA-C090 (kimA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2088 NBRP B. subtilis, Japan]
1A688 (''[[gene|kimA]]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]
GP93 (Δ[[gene|kimA]]::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
JH642 and its derivatives: natural deletion of the 18 kb [[gene|ydzA]]-[[gene|mntH]] region encompassing the genes [[gene|ydzA]]-[[gene|topB]]-[[gene|ydaJ]]-[[gene|ydaK]]-[[gene|ydaL]]-[[gene|ydaM]]-[[gene|ydaN]]-[[gene|kimA]]-[[gene|mutT]]-[[gene|ydaP]]-[[gene|ydzK]]-[[gene|mntH ]][pubmed|18670626]
BKE04320 (Δ[[gene|kimA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGATGTCTTCCCCTTTT, downstream forward: _UP4_TAAAGCTCTAGGACCAAGGG
BKK04320 (Δ[[gene|kimA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGATGTCTTCCCCTTTT, downstream forward: _UP4_TAAAGCTCTAGGACCAAGGG
GP2720 (Δ[[gene|kimA]]::''spc''), available in [SW|Jörg Stülke]'s lab
GP2721 (Δ[[gene|kimA]]::''ermC''), available in [SW|Jörg Stülke]'s lab
GP2498 (Δ[[gene|kimA]]::''cat'' [[gene|ktrA]]-[[gene|ktrB]]::''spc''), available in [SW|Jörg Stülke]'s lab
Biological materials
Expression vector
pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
Biological materials
lacZ fusion
GP2181 (based on [SW|pAC6]), available in [SW|Jörg Stülke]'s lab
BP144 (based on [SW|pAC5]), available in [SW|Fabian Commichau]' and [SW|Jörg Stülke]'s labs [pubmed|28420751]
Biological materials
FLAG-tag construct
GP2405 ''ydaO-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
GP2405 ''ydaO-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
References
Reviews
References
Original publications
proteinLength
607
geneLength
1824
Gene
Coordinates
486,432 488,255
Gene
Phenotypes of a mutant
a [[gene|kimA]] [[gene|ktrA]] [[gene|ktrB]] mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source [pubmed|28420751,28679749]
The protein
Catalyzed reaction/ biological activity
K+/H+ symport [pubmed|32005818,28420751]
The protein
Protein family
KUP family (single member) [pubmed|32005818]
[SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
Kinetic information
KM: 215 µM [pubmed|32005818]
Vmax 245 nmol min−1 mg−1 [pubmed|32005818]
The protein
[SW|Domains]
12 trans-membrane helices at the N-terminus [pubmed|32005818]
C-terminal cytoplasmic domain [pubmed|28420751]
The protein
Effectors of protein activity
activity is inhibited upon binding of c-di-AMP [pubmed|31061098]
in the Listeria monocytogenes protein, c-di-AMP binds to the C-terminal cytoplasmic domain of KimA [pubmed|31506295]
The protein
Structure
[PDB|6S3K] [pubmed|32005818]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Expression vectors
pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]
labs
[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]
[SW|Inga Hänelt], Frankfurt, Germany [https://www.biochem.uni-frankfurt.de/index.php?id=298 Homepage]