SubtiBank SubtiBank
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Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)2025-05-16 08:14:43

title

ydaO

kimA

description

putative transporter

high-affinity potassium transporter (uptake)

locus

BSU04320

BSU_04320

function

unknown

potassium uptake

product

unknown

high-affinity K+/H+ symporter

ec

names

ydaO

kimA

ydaO

outlinks

bsu

BSU04320

BSU_04320

[SW|Categories] containing this gene/protein

[SW|membrane proteins], [SW|proteins of unknown function]

Gene

Coordinates on the chromosome (coding sequence)

486,432 -> 488,255

The protein

[SW|Localization]

cell membrane (according to [http://www.uniprot.org/uniprot/P96589 UniProt])

cell membrane [pubmed|30452022]

Expression and Regulation

Operon

''[[protein|ydaO]]'' [Pubmed|22383849]

Expression and Regulation

Regulatory mechanism

expression is controlled via termination antitermination by the [SW|ydaO riboswitch], expression is switched off upon binding od c-di-AMP [Pubmed|24141192,20511502]

Biological materials

Mutant

1A688 (''[[protein|ydaO]]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]

GP93 (''[[protein|ydaO]]''::''cat''), available in [SW|Jörg Stülke]'s lab

MGNA-C090 (kimA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2088 NBRP B. subtilis, Japan]

1A688 (''[[gene|kimA]]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]

GP93 (Δ[[gene|kimA]]::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

JH642 and its derivatives: natural deletion of the 18 kb [[gene|ydzA]]-[[gene|mntH]] region encompassing the genes [[gene|ydzA]]-[[gene|topB]]-[[gene|ydaJ]]-[[gene|ydaK]]-[[gene|ydaL]]-[[gene|ydaM]]-[[gene|ydaN]]-[[gene|kimA]]-[[gene|mutT]]-[[gene|ydaP]]-[[gene|ydzK]]-[[gene|mntH ]][pubmed|18670626]

BKE04320 (Δ[[gene|kimA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGATGTCTTCCCCTTTT, downstream forward: _UP4_TAAAGCTCTAGGACCAAGGG

BKK04320 (Δ[[gene|kimA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGATGTCTTCCCCTTTT, downstream forward: _UP4_TAAAGCTCTAGGACCAAGGG

GP2720 (Δ[[gene|kimA]]::''spc''), available in [SW|Jörg Stülke]'s lab

GP2721 (Δ[[gene|kimA]]::''ermC''), available in [SW|Jörg Stülke]'s lab

GP2498 (Δ[[gene|kimA]]::''cat'' [[gene|ktrA]]-[[gene|ktrB]]::''spc''), available in [SW|Jörg Stülke]'s lab

Biological materials

Expression vector

pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

Biological materials

lacZ fusion

GP2181 (based on [SW|pAC6]), available in [SW|Jörg Stülke]'s lab

BP144 (based on [SW|pAC5]), available in [SW|Fabian Commichau]' and [SW|Jörg Stülke]'s labs [pubmed|28420751]

Biological materials

FLAG-tag construct

GP2405 ''ydaO-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

GP2405 ''ydaO-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

References

Reviews

25869574

25869574, 28825218, 32472931, 32603625

References

Original publications

15096624, 20511502, 23086297, 24141192, 25086509, 25086507

15096624, 20511502, 23086297, 24141192, 25086509, 25086507, 28420751, 28679749, 30452022, 31061098, 31506295, 32005818, 32253343

proteinLength

607

geneLength

1824

Gene

Coordinates

486,432 488,255

Gene

Phenotypes of a mutant

a [[gene|kimA]] [[gene|ktrA]] [[gene|ktrB]] mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source [pubmed|28420751,28679749]

The protein

Catalyzed reaction/ biological activity

K+/H+ symport [pubmed|32005818,28420751]

The protein

Protein family

KUP family (single member) [pubmed|32005818]

[SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

Kinetic information

KM: 215 µM [pubmed|32005818]

Vmax 245 nmol min−1 mg−1 [pubmed|32005818]

The protein

[SW|Domains]

12 trans-membrane helices at the N-terminus [pubmed|32005818]

C-terminal cytoplasmic domain [pubmed|28420751]

The protein

Effectors of protein activity

activity is inhibited upon binding of c-di-AMP [pubmed|31061098]

in the Listeria monocytogenes protein, c-di-AMP binds to the C-terminal cytoplasmic domain of KimA [pubmed|31506295]

The protein

Structure

[PDB|6S3K] [pubmed|32005818]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Expression vectors

pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

labs

[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]

[SW|Inga Hänelt], Frankfurt, Germany [https://www.biochem.uni-frankfurt.de/index.php?id=298 Homepage]