SubtiBank SubtiBank
Version comparison:

2017-05-09 15:36:352025-05-14 14:31:19

locus

BSU04320

BSU_04320

geneLength

1821

1824

product

high-affinity potassium transporter

high-affinity K+/H+ symporter

outlinks

bsu

BSU04320

BSU_04320

Gene

Coordinates

486,432 → 488,255

486,432 488,255

Gene

Phenotypes of a mutant

a [[gene|kimA ]][[gene|ktrA ]][[gene|ktrB ]]mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source [pubmed|28420751]

a [[gene|kimA]] [[gene|ktrA]] [[gene|ktrB]] mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source [pubmed|28420751,28679749]

The protein

Catalyzed reaction/ biological activity

uptake of potassium [pubmed|28420751]

K+/H+ symport [pubmed|32005818,28420751]

The protein

[SW|Domains]

11 trans-membrane helices at the N-terminus [pubmed|28420751]

12 trans-membrane helices at the N-terminus [pubmed|32005818]

C-terminal cytoplasmic domain [pubmed|28420751]

The protein

[SW|Localization]

cell membrane (according to [http://www.uniprot.org/uniprot/P96589 UniProt])

cell membrane [pubmed|30452022]

Biological materials

Mutant

MGNA-C090 (kimA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2088 NBRP B. subtilis, Japan]

1A688 (''[[gene|kimA]]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]

GP93 (''[[gene|kimA]]''::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

MGNA-C090 (kimA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2088 NBRP B. subtilis, Japan]

1A688 (''[[gene|kimA]]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]

GP93 (Δ[[gene|kimA]]::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

JH642 and its derivatives: natural deletion of the 18 kb [[gene|ydzA]]-[[gene|mntH]] region encompassing the genes [[gene|ydzA]]-[[gene|topB]]-[[gene|ydaJ]]-[[gene|ydaK]]-[[gene|ydaL]]-[[gene|ydaM]]-[[gene|ydaN]]-[[gene|kimA]]-[[gene|mutT]]-[[gene|ydaP]]-[[gene|ydzK]]-[[gene|mntH ]][pubmed|18670626]

BKE04320 (Δ[[gene|kimA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGATGTCTTCCCCTTTT, downstream forward: _UP4_TAAAGCTCTAGGACCAAGGG

BKK04320 (Δ[[gene|kimA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGATGTCTTCCCCTTTT, downstream forward: _UP4_TAAAGCTCTAGGACCAAGGG

GP2720 (Δ[[gene|kimA]]::''spc''), available in [SW|Jörg Stülke]'s lab

GP2721 (Δ[[gene|kimA]]::''ermC''), available in [SW|Jörg Stülke]'s lab

GP2498 (Δ[[gene|kimA]]::''cat'' [[gene|ktrA]]-[[gene|ktrB]]::''spc''), available in [SW|Jörg Stülke]'s lab

Biological materials

Expression vector

pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

Reviews

25869574

25869574, 28825218, 32472931, 32603625

References

Original publications

15096624, 20511502, 23086297, 24141192, 25086509, 25086507, 28420751

15096624, 20511502, 23086297, 24141192, 25086509, 25086507, 28420751, 28679749, 30452022, 31061098, 31506295, 32005818, 32253343

The protein

Protein family

KUP family (single member) [pubmed|32005818]

[SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

Kinetic information

KM: 215 µM [pubmed|32005818]

Vmax 245 nmol min−1 mg−1 [pubmed|32005818]

The protein

Effectors of protein activity

activity is inhibited upon binding of c-di-AMP [pubmed|31061098]

in the Listeria monocytogenes protein, c-di-AMP binds to the C-terminal cytoplasmic domain of KimA [pubmed|31506295]

The protein

Structure

[PDB|6S3K] [pubmed|32005818]

Biological materials

Expression vectors

pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

labs

[SW|Jörg Stülke], University of Göttingen, Germany [http://genmibio.uni-goettingen.de Homepage]

[SW|Inga Hänelt], Frankfurt, Germany [https://www.biochem.uni-frankfurt.de/index.php?id=298 Homepage]