SubtiBank SubtiBank
Version comparison:

2018-03-17 16:57:512025-05-13 11:14:39

locus

BSU29140

BSU_29140

geneLength

1116

1119

outlinks

bsu

BSU29140

BSU_29140

Gene

Coordinates

2,981,151 → 2,982,269

2,981,151 2,982,269

The protein

Catalyzed reaction/ biological activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA (according to Swiss-Prot)

acetyl-CoA + H2O + oxaloacetate --> citrate + CoA + H+ (according to UniProt)

The protein

Protein family

citrate synthase family (according to Swiss-Prot)

citrate synthase family (with [[protein|MmgD]] and [[protein|CitA]], according to UniProt)

Biological materials

Mutant

GP678 (erm), GP797 (spec) available in [SW|Jörg Stülke]'s lab

1A999 (''citZ''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A999&Search=1A999 BGSC]

GP790 (''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]''::''kan''), available in [SW|Jörg Stülke]'s lab

GP797 (''citZ''::''spec''), allows expression of ''[[gene|icd]]'' and ''[[gene|mdh]]'', available in [SW|Jörg Stülke]'s lab

GP1281 (''citZ''::''erm''), allows expression of ''[[gene|icd]]'' and ''[[gene|mdh]]'', available in [SW|Jörg Stülke]'s lab

GP2331 (''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]''::''kan''), Cre-recombinase is integrated in ''[[gene|sacA]]'',available in [SW|Jörg Stülke]'s lab

GP2333 (''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]''::''lox72''), Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab

BKE29140 (Δ[[gene|citZ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAACATCTCCTTTT, downstream forward: _UP4_TAAAGAACCATTGGAGGCTG

BKK29140 (Δ[[gene|citZ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAACATCTCCTTTT, downstream forward: _UP4_TAAAGAACCATTGGAGGCTG

GP678 (erm), GP797 (spec) available in [SW|Jörg Stülke]'s lab

1A999 (''citZ''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A999&Search=1A999 BGSC]

GP790 Δ(''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]'')::''kan'', available in [SW|Jörg Stülke]'s lab

GP797 (''citZ''::''spec''), allows expression of ''[[gene|icd]]'' and ''[[gene|mdh]]'', available in [SW|Jörg Stülke]'s lab

GP1281 (''citZ''::''erm''), allows expression of ''[[gene|icd]]'' and ''[[gene|mdh]]'', available in [SW|Jörg Stülke]'s lab

GP2331 Δ(''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]'')::''kan'', Cre-recombinase is integrated in ''[[gene|sacA]]'',available in [SW|Jörg Stülke]'s lab

GP2333 Δ(''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]'')::''lox72'', Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab

BKE29140 ([[gene|citZ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAACATCTCCTTTT, downstream forward: _UP4_TAAAGAACCATTGGAGGCTG

BKK29140 ([[gene|citZ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAACATCTCCTTTT, downstream forward: _UP4_TAAAGAACCATTGGAGGCTG

Biological materials

Expression vector

pGP1120 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab) [pubmed|20933603]

pGP1776 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab)

pGP1761 (expression with N-terminal His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP2515 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

pGP2261 (integration into [[gene|ganA]], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [SW|pGP888]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

[SW|Jörg Stülke], University of Göttingen, Germany

[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

References

Original publications

10348849, 8045899, 22900538, 10656796, 12850135, 17218307, 12100558, 9642180, 8045898, 8655569, 4211224, 4980242, 20525796, 24825009, 20933603, 23354745, 24571712

10348849, 8045899, 22900538, 10656796, 12850135, 17218307, 12100558, 9642180, 8045898, 8655569, 4211224, 4980242, 20525796, 24825009, 20933603, 23354745, 24571712, 29546354

Biological materials

Expression vectors

pGP1120 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab) [pubmed|20933603]

pGP1776 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab)

pGP1761 (expression with N-terminal His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

pGP2515 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

pGP2261 (integration into [[gene|ganA]], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [SW|pGP888]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]