2018-03-17 16:57:512025-05-13 11:14:39
locus
BSU29140
BSU_29140
geneLength
1116
1119
outlinks
bsu
BSU29140
BSU_29140
Gene
Coordinates
2,981,151 → 2,982,269
2,981,151 2,982,269
The protein
Catalyzed reaction/ biological activity
Acetyl-CoA + H2O + oxaloacetate = citrate + CoA (according to Swiss-Prot)
acetyl-CoA + H2O + oxaloacetate --> citrate + CoA + H+ (according to UniProt)
The protein
Protein family
citrate synthase family (according to Swiss-Prot)
citrate synthase family (with [[protein|MmgD]] and [[protein|CitA]], according to UniProt)
Biological materials
Mutant
GP678 (erm), GP797 (spec) available in [SW|Jörg Stülke]'s lab
1A999 (''citZ''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A999&Search=1A999 BGSC]
GP790 (''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]''::''kan''), available in [SW|Jörg Stülke]'s lab
GP797 (''citZ''::''spec''), allows expression of ''[[gene|icd]]'' and ''[[gene|mdh]]'', available in [SW|Jörg Stülke]'s lab
GP1281 (''citZ''::''erm''), allows expression of ''[[gene|icd]]'' and ''[[gene|mdh]]'', available in [SW|Jörg Stülke]'s lab
GP2331 (''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]''::''kan''), Cre-recombinase is integrated in ''[[gene|sacA]]'',available in [SW|Jörg Stülke]'s lab
GP2333 (''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]''::''lox72''), Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab
BKE29140 (Δ[[gene|citZ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAACATCTCCTTTT, downstream forward: _UP4_TAAAGAACCATTGGAGGCTG
BKK29140 (Δ[[gene|citZ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAACATCTCCTTTT, downstream forward: _UP4_TAAAGAACCATTGGAGGCTG
GP678 (erm), GP797 (spec) available in [SW|Jörg Stülke]'s lab
1A999 (''citZ''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A999&Search=1A999 BGSC]
GP790 Δ(''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]'')::''kan'', available in [SW|Jörg Stülke]'s lab
GP797 (''citZ''::''spec''), allows expression of ''[[gene|icd]]'' and ''[[gene|mdh]]'', available in [SW|Jörg Stülke]'s lab
GP1281 (''citZ''::''erm''), allows expression of ''[[gene|icd]]'' and ''[[gene|mdh]]'', available in [SW|Jörg Stülke]'s lab
GP2331 Δ(''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]'')::''kan'', Cre-recombinase is integrated in ''[[gene|sacA]]'',available in [SW|Jörg Stülke]'s lab
GP2333 Δ(''[[gene|citZ]]-[[gene|icd]]-[[gene|mdh]]'')::''lox72'', Cre-recombinase is integrated in ''[[gene|sacA]]'', available in [SW|Jörg Stülke]'s lab
BKE29140 ([[gene|citZ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAACATCTCCTTTT, downstream forward: _UP4_TAAAGAACCATTGGAGGCTG
BKK29140 ([[gene|citZ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATAACATCTCCTTTT, downstream forward: _UP4_TAAAGAACCATTGGAGGCTG
Biological materials
Expression vector
pGP1120 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab) [pubmed|20933603]
pGP1776 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab)
pGP1761 (expression with N-terminal His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
pGP2515 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
pGP2261 (integration into [[gene|ganA]], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [SW|pGP888]), available in [SW|Jörg Stülke]'s lab
Labs working on this gene/protein
[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
[SW|Jörg Stülke], University of Göttingen, Germany
[http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
References
Original publications
Biological materials
Expression vectors
pGP1120 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab) [pubmed|20933603]
pGP1776 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab)
pGP1761 (expression with N-terminal His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
pGP2515 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
pGP2261 (integration into [[gene|ganA]], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [SW|pGP888]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
[SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]