SubtiBank SubtiBank
Version comparison:

2017-12-11 13:33:142025-05-09 23:51:20

description

glycine cleavage system protein H, 2-oxo acid dehydrogenase, lipoyl-binding site, octanoyltransferase for lipoic acid biosynthesis

glycine cleavage system protein H, lipoyl-binding site, octanoyltransferase for lipoic acid biosynthesis, required for lipoate transfer to 2-oxo acid dehydrogenases

locus

BSU32800

BSU_32800

geneLength

381

384

outlinks

bsu

BSU32800

BSU_32800

Gene

Coordinates

3,366,123 → 3,366,506

3,366,123 3,366,506

The protein

Protein family

gcvH family (according to Swiss-Prot)

gcvH family (single member, according to UniProt)

Biological materials

Mutant

MGNA-B592 (yusH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1591 NBRP B. subtilis, Japan]

BKE32800 (Δ[[gene|gcvH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA

BKK32800 (Δ[[gene|gcvH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA

MGNA-B592 (yusH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1591 NBRP B. subtilis, Japan]

BKE32800 ([[gene|gcvH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA

BKK32800 ([[gene|gcvH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA

GP2585 ([[gene|gcvH]]::tet comIQ12L) (in DK1042) available in [SW|Jörg Stülke]'s lab

References

Original publications

20882995, 21338421, 20364333, 28900027

20882995, 21338421, 20364333, 28900027, 29339506, 31066113

The protein

Catalyzed reaction/ biological activity

degradation of glycine. [[protein|GcvH]] shuttles the methylamine group of glycine from [[protein|GcvPB]] to [[protein|GcvT]] (according to UniProt)

assembly of lipoate, and transfer of the cofactor to [[protein|PdhC]], [[protein|OdhB]], [[protein|AcoC]], and [[protein|BkdB]]

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|Lipoyl-binding domain] (aa 22-104) (according to UniProt)

Biological materials

Expression vector

pGP2328: in [SW|pBQ200], for expression in B. subtilis, available in [SW|Jörg Stülke]'s lab