2017-12-11 13:33:142025-05-09 23:51:20
description
glycine cleavage system protein H, 2-oxo acid dehydrogenase, lipoyl-binding site, octanoyltransferase for lipoic acid biosynthesis
glycine cleavage system protein H, lipoyl-binding site, octanoyltransferase for lipoic acid biosynthesis, required for lipoate transfer to 2-oxo acid dehydrogenases
locus
BSU32800
BSU_32800
geneLength
381
384
outlinks
bsu
BSU32800
BSU_32800
Gene
Coordinates
3,366,123 → 3,366,506
3,366,123 3,366,506
The protein
Protein family
gcvH family (according to Swiss-Prot)
gcvH family (single member, according to UniProt)
Biological materials
Mutant
MGNA-B592 (yusH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1591 NBRP B. subtilis, Japan]
BKE32800 (Δ[[gene|gcvH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
BKK32800 (Δ[[gene|gcvH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
MGNA-B592 (yusH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1591 NBRP B. subtilis, Japan]
BKE32800 ([[gene|gcvH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
BKK32800 ([[gene|gcvH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTGAATCCCTCCATTTT, downstream forward: _UP4_TAAAACAGGATAGCCGTAAA
GP2585 ([[gene|gcvH]]::tet comIQ12L) (in DK1042) available in [SW|Jörg Stülke]'s lab
References
Original publications
The protein
Catalyzed reaction/ biological activity
degradation of glycine. [[protein|GcvH]] shuttles the methylamine group of glycine from [[protein|GcvPB]] to [[protein|GcvT]] (according to UniProt)
assembly of lipoate, and transfer of the cofactor to [[protein|PdhC]], [[protein|OdhB]], [[protein|AcoC]], and [[protein|BkdB]]
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|Lipoyl-binding domain] (aa 22-104) (according to UniProt)
Biological materials
Expression vector
pGP2328: in [SW|pBQ200], for expression in B. subtilis, available in [SW|Jörg Stülke]'s lab