Tue Dec 13 2016 11:16:35 GMT+0100 (CET)2025-05-13 21:34:52
title
msmR
melR
description
transcriptional regulator of the msmR-msmE-amyD-amyC-melA operon
transcriptional regulator of the [[gene|melR]]-[[gene|melE]]-[[gene|melD]]-[[gene|melC]]-[[gene|melA]] operon
locus
BSU30260
BSU_30260
product
transcriptional regulator (LacI family)
transcriptional regulator ([SW|LacI family])
ec
names
msmR
melR
msmR
outlinks
bsu
BSU30260
BSU_30260
aminos
MVRIKDIALKAKVSSATVSRILNEDESLSVAGETRQRVINIAEELGYQTVAKRRKSRGQKQRAQPLIGVLSCLSPDQERQDPYFSSIRKGIEKECFEQEIFITNSIHLGSFQEHIFRELDGVIVIGRVHDEAVKHISGRLEHAVFINHSPDPQAYDSIGIDFESASRQAIDHLFDLGYKRLGYIGGQEKEHTLKDGQSIRRTIEDKRLTAFLESAAPQPEHVLIGEYSMREGYRLMKKAIDQGHLPEAFFIASDSMAIGALKALQEAGLQVPRDTAIVSFNGIEEAEFASTPLTTVKVYTEEMGRTGVKLLLDRLNGRTLPQHVTLPTTLIVRQSCGCTAKEVT
[SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources], [SW|transcription factors and their control]
Gene
Coordinates on the chromosome (coding sequence)
3,096,782 -> 3,097,816
Expression and Regulation
Operon
''[[protein|msmR]]-[[protein|msmE]]-[[protein|amyD]]-[[protein|amyC]]-[[protein|melA]]'' [Pubmed|22383849]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|22900538]
Expression and Regulation
Regulation
[[protein|MsmR]]
repressed by glucose ([[protein|CcpA]]) [Pubmed|22900538]
Expression and Regulation
Regulatory mechanism
[[protein|CcpA]]: transcription repression [Pubmed|22900538]
References
proteinLength
344
geneLength
1035
Gene
Coordinates
3,096,782 3,097,816
The protein
Catalyzed reaction/ biological activity
represses the the [[gene|melR]]-[[gene|melE]]-[[gene|melD]]-[[gene|melC]]-[[gene|melA]] operon in the absence of melibiose or raffinose [pubmed|31138628]
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|HTH lacI-type domain] (aa 2-58) (according to UniProt)
The protein
Structure
[PDB|1VPW] (E. coli [[protein|PurR]], 29% identity) [pubmed|9628480]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A802 (msmR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/802 NBRP B. subtilis, Japan]
BKE30260 ([[gene|melR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCCCTCTCATCT, downstream forward: _UP4_TAAGCAAAGATTCATCACGA
BKK30260 ([[gene|melR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGGTATCCCTCTCATCT, downstream forward: _UP4_TAAGCAAAGATTCATCACGA