Thu Jan 14 2016 18:41:57 GMT+0100 (CET)2025-05-29 20:12:52
locus
BSU06520
BSU_06520
product
phosphoribosylaminoimidazole carboxamide formyltransferase
and inosine-monophosphate cyclohydrolase
phosphoribosylaminoimidazole carboxamide formyltransferaseand inosine-monophosphate cyclohydrolase
outlinks
bsu
BSU06520
BSU_06520
[SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of nucleotides], [SW|most abundant proteins]
Gene
Coordinates on the chromosome (coding sequence)
708,594 -> 710,132
The protein
Catalyzed reaction/ biological activity
10-formyltetrahydrofolate 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to Swiss-Prot)
(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide --> (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)
H2O + IMP --> 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)
The protein
Protein family
purH family (according to Swiss-Prot)
purH family (single member, according to UniProt)
Expression and Regulation
Operon
''[[protein|purE]]-[[protein|purK]]-[[protein|purB]]-[[protein|purC]]-[[protein|purS]]-[[protein|purQ]]-[[protein|purL]]-[[protein|purF]]-[[protein|purM]]-[[protein|purN]]-[[protein|purH]]-[[protein|purD]]'' [Pubmed|3036807]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|3036807]
Expression and Regulation
Regulation
repressed in the presence of purine of purine nucleotides ([[protein|PurR]]) [Pubmed|7638212]
repressed in the presence of adenine or adenosine([[protein|PurR]]) [Pubmed|2536750]
repressed in the presence of guanine([[protein|G-box]]) [Pubmed|3036807]
Expression and Regulation
Regulatory mechanism
[[protein|PurR]]: transcription repression [Pubmed|7638212]
[[protein|PurR]]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750]
[[protein|G-box]]: transcription termination/ antitermination ([SW|riboswitch]) [http://www.ncbi.nlm.nih.gov/sites/entrez/3036807 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]
Expression and Regulation
Additional information
belongs to the 100 [[most abundant proteins]] [PubMed|15378759]
References
proteinLength
512
geneLength
1539
Gene
Coordinates
708,594 710,132
Gene
Phenotypes of a mutant
more resistant than wild-type to zinc limitation when grown on rich medium (LB), this is blocked by simultaneous deletion of [[gene|purB]] (reason: accumulation of ZTP that stimulates the binding of [[protein|ZagA]] to [[protein|FolE]], and thus folate biosynthesis during zinc limitation) [pubmed|31132310]
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
MGS-like domain (aa 1-146) (according to UniProt)
The protein
additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE06520 ([[gene|purH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA
BKK06520 ([[gene|purH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA