2017-8-11 16:0:32025-05-22 18:24:39
description
glycine betaine ABC transporter (binding protein)
glycine betaine and arsenobetaine [SW|ABC transporter] (binding protein)
locus
BSU03000
BSU_03000
geneLength
879
882
product
glycine betaine ABC transporter (binding protein)
glycine betaine and arsenobetaine [SW|ABC transporter] (binding protein)
ec
outlinks
bsu
BSU03000
BSU_03000
Gene
Coordinates
323,119 → 324,000
323,119 324,000
The protein
Catalyzed reaction/ biological activity
binding for subsequent uptake of glycine betaine [Pubmed|24561588]
binding for subsequent uptake of dimethylglycine [Pubmed|24561588]
binding for subsequent uptake of glycine betaine and arsenobetaine [pubmed|29159878,24561588]
binding for subsequent uptake of dimethylglycine [Pubmed|24561588]
The protein
Structure
[PDB|2B4L] (complex with glycine betaine), [PDB|3CHG] (in complex with DMSA)
[PDB|2B4L] (complex with glycine betaine)
[PDB|3CHG] (in complex with DMSA)
[PDB|5NXX] (in complex with arsenobetaine) [pubmed|29159878]
Biological materials
Expression vector
pGP2926 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
Labs working on this gene/protein
[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]
References
Original publications
The protein
Paralogous protein(s)
[[this]]
Biological materials
Mutant
BKE03000 ([[gene|opuAC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCCTATGATTTTTTTAA, downstream forward: _UP4_TAATCAAAAAAGCAGCCTGT
BKK03000 ([[gene|opuAC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCCTATGATTTTTTTAA, downstream forward: _UP4_TAATCAAAAAAGCAGCCTGT
Biological materials
Expression vectors
pGP2926 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]