Tue Dec 13 2016 11:16:33 GMT+0100 (CET)2025-05-30 14:56:21
locus
BSU18480
BSU_18480
outlinks
uniprot
P14383
P0CI77
outlinks
bsu
BSU18480
BSU_18480
aminos
45))BacillussubtilissubtilisMLPIFDQKKVAFIGAGSMAEGMISGIVRANKIPKQNICVTNRSNTERLTELELQYGIKGALPNQLCIEDMDVLILAMKPKDAENALSSLKSRIQPHQLILSVLAGITTSFIEQSLLNEQPVVRVMPNTSSMIGASATAIALGKYVSEDLKKLAEALLGCMGEVYTIQENQMDIFTGIAGSGPAYFYYLMEFIEKTGEEAGLDKQLSRSIGAQTLLGAAKMLMETGEHPEILRDNITSPNGTTAAGLQALKKSGGGEAISQAIKHAAKRSKEISEDIEKTAAPLSGVIK
[SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of amino acids], [SW|coping with hyper-osmotic stress]
Gene
Coordinates on the chromosome (coding sequence)
2,016,845 -> 2,017,738
The protein
Catalyzed reaction/ biological activity
L-proline NAD(P) = 1-pyrroline-5-carboxylate NAD(P)H (according to Swiss-Prot)
1-pyrroline-5-carboxylate + NAD(P)H + H+ --> L-proline + NAD(P)+ [pubmed|28824574]
The protein
Protein family
glutamate 5-kinase family (according to Swiss-Prot)
[SW|pyrroline-5-carboxylate reductase family] [pubmed|28824574]
The protein
Paralogous protein(s)
[[protein|ProI]]
[[this]]
The protein
Structure
[PDB|5BSE] (from ''Medicago trunculata'', 36% identity)
[PDB|5BSE] (from ''Medicago truncatula'', 36% identity) [pubmed|26579138]
Expression and Regulation
Operon
''[[protein|proH]]-[[protein|proJ]]'' [Pubmed|21296969]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|21784929]
Expression and Regulation
Regulation
expressed under conditions of osmotic stress [Pubmed|21784929]
induced by cold stress [Pubmed|21296969]
expression during spore [SW|germination] is increased under conditions of osmotic stress [Pubmed|27766092]
References
Original publications
proteinLength
297
geneLength
894
Gene
Coordinates
2,016,845 2,017,738
The protein
[SW|Cofactors]
NADPH [pubmed|28824574]
The protein
Effectors of protein activity
feedback-inhibited by proline [pubmed|28824574]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
HB24914 ([[gene|proH]]::''erm''), available in [SW|John Helmann]'s and [SW|Jörg Stülke]'s labs
MGNA-B083 (proH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1082 NBRP B. subtilis, Japan]
BKE18480 ([[gene|proH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCACACGTTTAAAAT, downstream forward: _UP4_GCGCCGCTATCAGGAGTGAT
BKK18480 ([[gene|proH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCACACGTTTAAAAT, downstream forward: _UP4_GCGCCGCTATCAGGAGTGAT