SubtiBank SubtiBank
Version comparison:

2018-10-21 18:18:542025-05-24 04:54:07

locus

BSU14560

BSU_14560

geneLength

552

555

outlinks

bsu

BSU14560

BSU_14560

Gene

Coordinates

1,526,195 → 1,526,749

1,526,195 1,526,749

The protein

Catalyzed reaction/ biological activity

N-acetylcysteine -→ cysteine + acetate [Pubmed|23944997]

N-acetylcysteine - cysteine + acetate [Pubmed|23944997]

H2O + N-terminal N-formyl-L-methionyl-[peptide] --> formate + N-terminal L-methionyl-[peptide] (according to UniProt)

The protein

Protein family

polypeptide deformylase family (according to Swiss-Prot)

polypeptide deformylase family (with [[protein|DefA]], according to UniProt)

The protein

Structure

[PDB|1LQY] (complex with antibiotic actinonin, Geobacillus stearothermophilus)

[PDB|1LQY] (complex with antibiotic actinonin, Geobacillus stearothermophilus, 67% identity) [pubmed|12126617]

Biological materials

Mutant

MGNA-A901 (ykrB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/901 NBRP B. subtilis, Japan]

BKE14560 (Δ[[gene|defB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGAAACTTCACTCCTAAA, downstream forward: _UP4_TAAAAGAGAAAACGGCTGGC

BKK14560 (Δ[[gene|defB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGAAACTTCACTCCTAAA, downstream forward: _UP4_TAAAAGAGAAAACGGCTGGC

MGNA-A901 (ykrB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/901 NBRP B. subtilis, Japan]

BKE14560 ([[gene|defB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGAAACTTCACTCCTAAA, downstream forward: _UP4_TAAAAGAGAAAACGGCTGGC

BKK14560 ([[gene|defB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGAAACTTCACTCCTAAA, downstream forward: _UP4_TAAAAGAGAAAACGGCTGGC

References

11429456, 23944997, 26735940, 27983482

11429456, 23944997, 26735940, 27983482, 12126617