Fri Dec 09 2016 17:22:59 GMT+0100 (CET)2025-05-09 22:28:36
description
major high-affinity Na -coupled glutamate/ aspartate symport protein
major high-affinity Na+-coupled glutamate/ aspartate symport protein, uptake of glyphosate
locus
BSU10220
BSU_10220
product
major Na -coupled glutamate/ aspartate symport protein
major Na+-coupled glutamate/ aspartate symport protein
ec
outlinks
bsu
BSU10220
BSU_10220
[SW|Categories] containing this gene/protein
[SW|transporters/ other], [SW|biosynthesis/ acquisition of amino acids], [SW|glutamate metabolism], [SW|utilization of amino acids], [SW|membrane proteins]
Gene
Coordinates on the chromosome (coding sequence)
1,096,560 -> 1,097,849
The protein
Protein family
View classification (according to Swiss-Prot)
[SW|dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|GltP]], [[protein|DctP]]
[[this]]
Expression and Regulation
Operon
''gltT'' [Pubmed|22383849]
Biological materials
Mutant
available in [SW|Erhard Bremer]'s lab [Pubmed|25344233]
BP233 (Δ[[gene|gltT]]::''spc''), available in [SW|Fabian Commichau]'s lab
BP235 (Δ[[gene|gltT]]::''spc '' Δ[[gene|gltP]]::''cat''), available in [SW|Fabian Commichau]'s lab
GP2247 (Δ[[gene|gltT]]::''ermC''), available in [SW|Jörg Stülke]'s lab
GP2248 (Δ[[gene|gltT]]::''aphA3''), available in [SW|Jörg Stülke]'s lab
GP2722 (Δ[[gene|gltT]]::''zeo''), available in [SW|Jörg Stülke]'s lab
BKE10220 ([[gene|gltT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGATTACCTCCCAAAAA, downstream forward: _UP4_TAATGAAAAGCCTGCGGGGT
BKK10220 ([[gene|gltT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGATTACCTCCCAAAAA, downstream forward: _UP4_TAATGAAAAGCCTGCGGGGT
GP2831 (''Δ''''[[gene|gltT]]''::''aphA3'' ''Δ''''[[gene|aimA]]''::''phleo''), available in [SW|Jörg Stülke]'s lab
References
proteinLength
429
geneLength
1290
Gene
Coordinates
1,096,560 1,097,849
The protein
Catalyzed reaction/ biological activity
uptake of glutamate and aspartate
uptake of glyphosate and glufosinate [pubmed|30666812]
The protein
Kinetic information
Km values: 37 +/- 5 µM for glutamate, 41 +/- 9 µM for aspartate [pubmed|25344233]
The protein
Effectors of protein activity
aspartate uptake is competitively inhibited by glutamate [pubmed|29995990]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
FLAG-tag construct
GP3063 ''gltT-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
Biological materials
Expression vectors
for expression in ''B. subtilis'', in [SW|pAC7]: pBP56, available in [SW|Fabian Commichau]'s lab