major high-affinity Na⁺-coupled glutamate/ aspartate symport protein

major high-affinity Na⁺-coupled glutamate/ aspartate symport protein, uptake of glyphosate

BSU10220

BSU_10220

1287

1290

BSU10220

BSU_10220

1,096,560 → 1,097,849

1,096,560 1,097,849

View classification (according to Swiss-Prot)

[SW|dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family] (according to UniProt)

GP2247 (''[[gene|gltT]]''::''ermC''), available in [SW|Jörg Stülke]'s lab

GP2248 (''[[gene|gltT]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab

GP2722 (''[[gene|gltT]]''::''zeo''), available in [SW|Jörg Stülke]'s lab

available in [SW|Erhard Bremer]'s lab [Pubmed|25344233]

BKE10220 (Δ[[gene|gltT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGATTACCTCCCAAAAA, downstream forward: _UP4_TAATGAAAAGCCTGCGGGGT

BKK10220 (Δ[[gene|gltT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGATTACCTCCCAAAAA, downstream forward: _UP4_TAATGAAAAGCCTGCGGGGT

BP233 (Δ[[gene|gltT]]::''spc''), available in [SW|Fabian Commichau]'s lab

BP235 (Δ[[gene|gltT]]::''spc '' Δ[[gene|gltP]]::''cat''), available in [SW|Fabian Commichau]'s lab

GP2247 (Δ[[gene|gltT]]::''ermC''), available in [SW|Jörg Stülke]'s lab

GP2248 (Δ[[gene|gltT]]::''aphA3''), available in [SW|Jörg Stülke]'s lab

GP2722 (Δ[[gene|gltT]]::''zeo''), available in [SW|Jörg Stülke]'s lab

BKE10220 ([[gene|gltT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGATTACCTCCCAAAAA, downstream forward: _UP4_TAATGAAAAGCCTGCGGGGT

BKK10220 ([[gene|gltT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGATTACCTCCCAAAAA, downstream forward: _UP4_TAATGAAAAGCCTGCGGGGT

GP2831 (''Δ''''[[gene|gltT]]''::''aphA3'' ''Δ''''[[gene|aimA]]''::''phleo''), available in [SW|Jörg Stülke]'s lab

18763711, 23563139, 22383849, 24013209, 25344233

18763711, 23563139, 22383849, 24013209, 25344233, 14596613, 10588697, 29995990, 30666812

uptake of glutamate and aspartate

uptake of glyphosate and glufosinate [pubmed|30666812]

Km values: 37 +/- 5 µM for glutamate, 41 +/- 9 µM for aspartate [pubmed|25344233]

aspartate uptake is competitively inhibited by glutamate [pubmed|29995990]

GP3063 ''gltT-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

for expression in ''B. subtilis'', in [SW|pAC7]: pBP56, available in [SW|Fabian Commichau]'s lab