Mon Aug 31 2015 13:53:16 GMT+0200 (CEST)2025-05-14 01:42:56
description
biosynthesis of the pyrimidine moiety of thiamine
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase, biosynthesis of the pyrimidine moiety of thiamine
locus
BSU08790
BSU_08790
product
unknown
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase
ec
outlinks
bsu
BSU08790
BSU_08790
syns
thiA
[SW|Categories] containing this gene/protein
[SW|biosynthesis of cofactors], [SW|phosphoproteins]
The protein
Protein family
thiC family (according to Swiss-Prot)
thiC family (single member, according to UniProt)
The protein
Structure
[http://www.proteopedia.org/wiki/index.php/1g6c 1G6C]
[PDB|3EPN] (from Caulobacter crescentus, 65% identity) [pubmed|18953358]
Expression and Regulation
Operon
''[[protein|thiC]]'' [Pubmed|22383849]
Expression and Regulation
Regulation
repressed by thiamine and 2-methyl-4-amino-5-hydroxymethylpyrimidine [Pubmed|9370266]
repressed by thiamine ([[protein|Thi-box]]) [Pubmed|12376536]
Expression and Regulation
Regulatory mechanism
[[protein|Thi-box]]: [SW|RNA switch] [Pubmed|12376536]
Biological materials
Mutant
1A603 ( ''thiC''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A603&Search=1A603 BGSC]
1A603 ( ''thiC''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A603&Search=1A603 BGSC]
BKE08790 ([[gene|thiC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTTTTCCTTCCCCTT, downstream forward: _UP4_TAAAAAAAATGAAGATGGAG
BKK08790 ([[gene|thiC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTTTTCCTTCCCCTT, downstream forward: _UP4_TAAAAAAAATGAAGATGGAG
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
References
Original publications
proteinLength
590
geneLength
1773
Gene
Coordinates
955,895 957,667
The protein
Catalyzed reaction/ biological activity
5-amino-1-(5-phospho-β-D-ribosyl)imidazole + S-adenosyl-L-methionine --> 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H+ + L-methionine (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Cofactors]
Fe-S cluster [pubmed|29292548]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]