SubtiBank SubtiBank
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Mon Aug 31 2015 13:53:16 GMT+0200 (CEST)2025-05-14 01:42:56

description

biosynthesis of the pyrimidine moiety of thiamine

4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase, biosynthesis of the pyrimidine moiety of thiamine

locus

BSU08790

BSU_08790

product

unknown

4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate (HMP-P) synthase

ec

outlinks

bsu

BSU08790

BSU_08790

syns

thiA

[SW|Categories] containing this gene/protein

[SW|biosynthesis of cofactors], [SW|phosphoproteins]

The protein

Protein family

thiC family (according to Swiss-Prot)

thiC family (single member, according to UniProt)

The protein

Structure

[http://www.proteopedia.org/wiki/index.php/1g6c 1G6C]

[PDB|3EPN] (from Caulobacter crescentus, 65% identity) [pubmed|18953358]

Expression and Regulation

Operon

''[[protein|thiC]]'' [Pubmed|22383849]

Expression and Regulation

Regulation

repressed by thiamine and 2-methyl-4-amino-5-hydroxymethylpyrimidine [Pubmed|9370266]

repressed by thiamine ([[protein|Thi-box]]) [Pubmed|12376536]

Expression and Regulation

Regulatory mechanism

[[protein|Thi-box]]: [SW|RNA switch] [Pubmed|12376536]

Biological materials

Mutant

1A603 ( ''thiC''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A603&Search=1A603 BGSC]

1A603 ( ''thiC''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A603&Search=1A603 BGSC]

BKE08790 ([[gene|thiC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTTTTCCTTCCCCTT, downstream forward: _UP4_TAAAAAAAATGAAGATGGAG

BKK08790 ([[gene|thiC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACTTTTCCTTCCCCTT, downstream forward: _UP4_TAAAAAAAATGAAGATGGAG

Biological materials

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

Original publications

16291685, 9370266, 20509597, 12376536, 22517742, 22383849

16291685, 9370266, 20509597, 12376536, 22517742, 22383849, 18953358, 28516784, 29794222

proteinLength

590

geneLength

1773

Gene

Coordinates

955,895 957,667

The protein

Catalyzed reaction/ biological activity

5-amino-1-(5-phospho-β-D-ribosyl)imidazole + S-adenosyl-L-methionine --> 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H+ + L-methionine (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Cofactors]

Fe-S cluster [pubmed|29292548]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]