SubtiBank SubtiBank
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Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-29 20:10:57

description

heme-binding protein, essential for heme biosynthesis

coproheme decarboxylase

locus

BSU37670

BSU_37670

product

heme-binding protein

coproheme decarboxylase

outlinks

bsu

BSU37670

BSU_37670

aminos

MSEQQMTNEAAKTLDGWYALHDFRTMDWASWKLLSSDERQSIIHEFTGLLEKWGVAQKEGKGSQTLYSIVGQKADFMLMILRPTMEELNQIELEFNKSRLAEFTIPAYSYVSVVELSNYLASGDGDPYENPHVRARLYPELPESKYVCFYPMDKRRSGNDNWYMLSMEERRNLMRSHGLIGRSYAGKVKQIITGSVGFDDYEWGVTLFSDDVLQFKKLVYEMRFDEVSARYGEFGSFFVGNHLSLDTLPQFLYV

[SW|Categories] containing this gene/protein

[SW|biosynthesis of cofactors], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence)

3,866,596 -> 3,867,360

The protein

Protein family

UPF0447 family (according to Swiss-Prot)

UPF0447 family (single member, according to UniProt)

The protein

Structure

[PDB|1T0T] (''Geobacillus stearothermophilus'')

[PDB|5T2K] (''Geobacillus stearothermophilus'') [pubmed|27936663]

Expression and Regulation

Operon

''hemQ'' [Pubmed|9353933]

Biological materials

Expression vector

pGP2643: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab

pGP2644: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

pGP2637: N-terminal Strep-tag, in [SW|pGP380], for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

References

17218307, 9353933, 20543190

proteinLength

254

geneLength

765

Gene

Coordinates

3,866,596 3,867,360

The protein

Catalyzed reaction/ biological activity

coproheme --> protoheme + 2 CO2 + 4 H+ [pubmed|25646457]

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-A516 (ywfI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/516 NBRP B. subtilis, Japan]

BKK37670 ([[gene|hemQ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATCTTCACTCCCATT, downstream forward: _UP4_TAATATCCCCTCCCGCCCTA

Biological materials

Expression vectors

pGP2643: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab

pGP2644: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

pGP2637: N-terminal Strep-tag, in [SW|pGP380], for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

References

Reviews

28123057, 25711532

References

Original Publications

17218307, 9353933, 20543190, 25646457, 27758026, 27982566, 27599156, 27936663, 26083961, 27597779