Tue Dec 13 2016 11:16:37 GMT+0100 (CET)2025-05-29 20:10:57
description
heme-binding protein, essential for heme biosynthesis
coproheme decarboxylase
locus
BSU37670
BSU_37670
product
heme-binding protein
coproheme decarboxylase
outlinks
bsu
BSU37670
BSU_37670
aminos
MSEQQMTNEAAKTLDGWYALHDFRTMDWASWKLLSSDERQSIIHEFTGLLEKWGVAQKEGKGSQTLYSIVGQKADFMLMILRPTMEELNQIELEFNKSRLAEFTIPAYSYVSVVELSNYLASGDGDPYENPHVRARLYPELPESKYVCFYPMDKRRSGNDNWYMLSMEERRNLMRSHGLIGRSYAGKVKQIITGSVGFDDYEWGVTLFSDDVLQFKKLVYEMRFDEVSARYGEFGSFFVGNHLSLDTLPQFLYV
[SW|Categories] containing this gene/protein
[SW|biosynthesis of cofactors], [SW|phosphoproteins]
Gene
Coordinates on the chromosome (coding sequence)
3,866,596 -> 3,867,360
The protein
Protein family
UPF0447 family (according to Swiss-Prot)
UPF0447 family (single member, according to UniProt)
The protein
Structure
[PDB|1T0T] (''Geobacillus stearothermophilus'')
[PDB|5T2K] (''Geobacillus stearothermophilus'') [pubmed|27936663]
Expression and Regulation
Operon
''hemQ'' [Pubmed|9353933]
Biological materials
Expression vector
pGP2643: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab
pGP2644: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
pGP2637: N-terminal Strep-tag, in [SW|pGP380], for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
References
proteinLength
254
geneLength
765
Gene
Coordinates
3,866,596 3,867,360
The protein
Catalyzed reaction/ biological activity
coproheme --> protoheme + 2 CO2 + 4 H+ [pubmed|25646457]
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
MGNA-A516 (ywfI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/516 NBRP B. subtilis, Japan]
BKK37670 ([[gene|hemQ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATCTTCACTCCCATT, downstream forward: _UP4_TAATATCCCCTCCCGCCCTA
Biological materials
Expression vectors
pGP2643: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab
pGP2644: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
pGP2637: N-terminal Strep-tag, in [SW|pGP380], for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
References
Reviews
References
Original Publications