SubtiBank SubtiBank
Version comparison:

Tue Oct 13 2015 12:40:43 GMT+0200 (CEST)2025-04-07 04:33:42

description

ABC transporter for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein), with YusV as ATPase

[SW|ABC transporter] for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein), with [[protein|YusV]] as ATPase

locus

BSU01630

BSU_01630

function

acquisition of iron

[SW|acquisition of iron]

product

ABC transporter for the siderophores Fe-enterobactin
and Fe-bacillibactin (binding protein)

[SW|ABC transporter] for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein) [pubmed|28283524]

ec

outlinks

bsu

BSU01630

BSU_01630

[SW|Categories] containing this gene/protein

[SW|ABC transporters], [SW|acquisition of iron], [SW|iron metabolism], [SW|membrane proteins]

The protein

Protein family

Fe/B12 periplasmic-binding domain (according to Swiss-Prot)

[SW|Bacterial solute-binding protein 8 family] (according to UniProt)

The protein

[SW|Domains]

Two independent non-symmetric globular domains, connected by a long alpha-helix (residues 143-164 of the mature protein).

Two independent non-symmetric globular domains, connected by a long alpha-helix (residues 143-164 of the mature protein).

[SW|Fe/B12 periplasmic-binding domain] (aa 57-317) (according to UniProt)

The protein

Structure

[PDB|2PHZ], [PDB|2WI8], [PDB|2WHY] (complex with ferri-bacillibactin), [PDB|2XUZ] (complex with ferri-enterobactin), [PDB|2XV1] (complex with ferric mecam)

[PDB|2PHZ], [PDB|2WI8], [PDB|2WHY] (complex with ferri-bacillibactin)

[PDB|2XUZ] (complex with ferri-enterobactin)

[PDB|2XV1] (complex with ferric mecam)

The protein

[SW|Interactions]

[[protein|YusV]]-([[protein|FeuB]]-[[protein|FeuC]])-[[protein|FeuA]] [Pubmed|10092453]

Expression and Regulation

Operon

''[[protein|feuA]]-[[protein|feuB]]-[[protein|feuC]]-[[protein|ybbA]]'' [Pubmed|12354229]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|17725565]

Expression and Regulation

Regulation

repressed in the presence of iron ([[protein|Fur]], [[protein|CitB]]) [Pubmed|12354229]

induced in the presence of iron chelators bacillibactin or enterobactin ([[protein|Btr]]) [Pubmed|17725565]

Expression and Regulation

Regulatory mechanism

[[protein|Fur]]: transcription repression [Pubmed|12354229]

[[protein|CitB]]: binding to the iron responsive element in the absence of iron

[[protein|Btr]]: transcription activation in the presence of the co-activators bacillibactin or enterobactin [Pubmed|17725565]

References

20086155, 19746494, 19673474, 14563870, 16672620, 10092453, 16889643, 17725565, 12354229, 18957862, 18763711, 22303020

20086155, 19746494, 19673474, 14563870, 16672620, 10092453, 16889643, 17725565, 12354229, 18957862, 18763711, 22303020, 10468622, 28283524, 29133393, 31113899

proteinLength

317

geneLength

954

Gene

Coordinates

182,370 183,323

The protein

Paralogous protein(s)

[[this]]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE01630 ([[gene|feuA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAGAGCCTCCTGTT, downstream forward: _UP4_AACTAATTCAGAGTAGGTTT

BKK01630 ([[gene|feuA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATAGAGCCTCCTGTT, downstream forward: _UP4_AACTAATTCAGAGTAGGTTT

Biological materials

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab