2017-8-11 16:0:32025-05-12 05:30:46
title
mutTA
rppH
description
error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity
decapping of NAD-capped RNAs, error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity
locus
BSU30630
BSU_30630
geneLength
474
477
ec
names
mutTA
ytkD
rppH
mutTA
ytkD
outlinks
bsu
BSU30630
BSU_30630
Gene
Coordinates
3,134,983 → 3,135,459
3,134,983 3,135,459
The protein
Catalyzed reaction/ biological activity
removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by [SW|RNases] [Pubmed|21925382]
RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position [Pubmed|23610425,23610407]
removal of pyrophosphate from the 5' end of mRNAs to make RNA accessible for degradation by [SW|RNases] [Pubmed|21925382]
RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position [Pubmed|23610425,23610407]
decapping of NAD-capped RNAs [pubmed|30110644]
8-oxo-dGTP + H2O --> 8-oxo-dGMP + diphosphate + H+ (according to UniProt)
The protein
Protein family
[SW|Nudix hydrolase] family (according to Swiss-Prot)
[SW|Nudix hydrolase] (according to UniProt)
Biological materials
Mutant
MGNA-A297 (ytkD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/297 NBRP B. subtilis, Japan]
MGNA-A297 (ytkD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/297 NBRP B. subtilis, Japan]
BKE30630 ([[gene|rppH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCTCAAATCC, downstream forward: _UP4_TAAAAAACCCGGCACATGTG
BKK30630 ([[gene|rppH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCTCAAATCC, downstream forward: _UP4_TAAAAAACCCGGCACATGTG
References
Reviews
References
Original publications
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|Nudix hydrolase domain] (aa 6-145) (according to UniProt)