SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-12 05:30:46

title

mutTA

rppH

description

error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity

decapping of NAD-capped RNAs, error prevention oxidized guanine system, confers protection against oxidative stress to vegetative cells, moreover the protein has RNA pyrophosphohydrolase activity

locus

BSU30630

BSU_30630

geneLength

474

477

ec

names

mutTA

ytkD

rppH

mutTA

ytkD

outlinks

bsu

BSU30630

BSU_30630

Gene

Coordinates

3,134,983 → 3,135,459

3,134,983 3,135,459

The protein

Catalyzed reaction/ biological activity

removal of pyrophosphate from the 5' end of mRNAs to make the RNA accessible for degradation by [SW|RNases] [Pubmed|21925382]

RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position [Pubmed|23610425,23610407]

removal of pyrophosphate from the 5' end of mRNAs to make RNA accessible for degradation by [SW|RNases] [Pubmed|21925382]

RppH requires at least two unpaired nucleotides at the 5' end of its RNA substrates and prefers three or more. The second of these 5'-terminal nucleotides must be G, whereas a less strict preference for a purine is evident at the third position, and A is slightly favored over G at the first position [Pubmed|23610425,23610407]

decapping of NAD-capped RNAs [pubmed|30110644]

8-oxo-dGTP + H2O --> 8-oxo-dGMP + diphosphate + H+ (according to UniProt)

The protein

Protein family

[SW|Nudix hydrolase] family (according to Swiss-Prot)

[SW|Nudix hydrolase] (according to UniProt)

Biological materials

Mutant

MGNA-A297 (ytkD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/297 NBRP B. subtilis, Japan]

MGNA-A297 (ytkD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/297 NBRP B. subtilis, Japan]

BKE30630 ([[gene|rppH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCTCAAATCC, downstream forward: _UP4_TAAAAAACCCGGCACATGTG

BKK30630 ([[gene|rppH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCTCAAATCC, downstream forward: _UP4_TAAAAAACCCGGCACATGTG

References

Reviews

22568516

22568516, 32061882

References

Original publications

16513759, 16497325, 14761999, 19011023, 9387221, 15576788, 21925382, 23610425, 23610407

16513759, 16497325, 14761999, 19011023, 9387221, 15576788, 21925382, 23610425, 23610407, 30110644

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|Nudix hydrolase domain] (aa 6-145) (according to UniProt)