Fri Dec 04 2015 20:48:48 GMT+0100 (CET)2025-03-13 17:45:16
locus
BSU23270
BSU_23270
outlinks
bsu
BSU23270
BSU_23270
[SW|Categories] containing this gene/protein
[SW|biosynthesis of cofactors]
Gene
Coordinates on the chromosome (coding sequence)
2,429,600 -> 2,430,247
The protein
Catalyzed reaction/ biological activity
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine (according to Swiss-Prot)
2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H+ --> 5-amino-6-(D-ribitylamino)uracil + riboflavin (according to UniProt)
The protein
[SW|Interactions]
([[protein|RibE]])3-([[protein|RibH]])60, the lumazine synthase/riboflavin synthase complex [Pubmed|7473709]
Expression and Regulation
Operon
''[[protein|ribD]]-[[protein|ribE]]-[[protein|ribA]]-[[protein|ribH]]-[[protein|ribT]]'' [Pubmed|8159171]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|8159171]
Expression and Regulation
Regulation
expressed in the absence of FMN ([[protein|FMN-box]]) [Pubmed|15808508]
Expression and Regulation
Regulatory mechanism
[[protein|FMN-box]]: [SW|riboswitch], mediates termination of transcription in the presence of FMN or FMNH2, this is counter-acted upon binding of [SW|RibR] [Pubmed|26494285], [Pubmed|15808508]
Biological materials
Mutant
GP203 (erm), available in the [SW|Stülke] lab
GP203 (erm), available in [SW|Jörg Stülke]'s lab
BKE23270 ([[gene|ribE]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTCCTGTAAACATGGTCA, downstream forward: _UP4_GGCTTTTAGAGAGGAAGATT
BKK23270 ([[gene|ribE]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATTCCTGTAAACATGGTCA, downstream forward: _UP4_GGCTTTTAGAGAGGAAGATT
References
proteinLength
215
geneLength
648
Gene
Coordinates
2,429,600 2,430,247
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
References
Reviews
References
Original publications