2017-09-16 17:28:102025-05-12 03:19:30
description
oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase
oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase, degradation of pGpG
locus
BSU29250
BSU_29250
geneLength
939
942
outlinks
bsu
BSU29250
BSU_29250
Gene
Coordinates
2,995,908 → 2,996,849
2,995,908 2,996,849
The protein
Catalyzed reaction/ biological activity
oligoribonuclease (nanoRNAse), 3',5'-bisphosphate nucleotidase [Pubmed|17586819]
hydrolyzes oligo(deoxy)ribonucleotides in a 5'→ 3' direction [Pubmed|28894100,21087930]
hydrolyzes nano-RNAs in a 3'→ 5' direction [Pubmed|28894100]
oligoribonuclease (nanoRNAse), 3',5'-bisphosphate nucleotidase [Pubmed|17586819]
hydrolyzes oligo(deoxy)ribonucleotides in a 5' 3' direction [Pubmed|28894100,21087930]
hydrolyzes nano-RNAs in a 3' 5' direction [Pubmed|28894100]
degradation of pGpG derived from c-di-GMP [pubmed|30249708]
adenosine 3',5'-bisphosphate + H2O --> AMP + phosphate (according to UniProt)
The protein
[SW|Domains]
[SW|DHH-DHHA1 domain]
[SW|DHH-DHHA1 domain] [pubmed|28894100]
Biological materials
Mutant
MGNA-A151 (ytqI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/151 NBRP B. subtilis, Japan]
GP2155 (''[[gene|nrnA]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
BKE29250 (Δ[[gene|nrnA]]::erm trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU29250 BGSC], upstream reverse:
_UP1_CATTTGATACTCCTTTATTG, downstream forward:
_UP4_GAGTAGAGGGGAGACCCTCT
BKK29250 (Δ[[gene|nrnA]]::kan trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU29250 BGSC], upstream reverse:
_UP1_CATTTGATACTCCTTTATTG, downstream forward:
_UP4_GAGTAGAGGGGAGACCCTCT
MGNA-A151 (ytqI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/151 NBRP B. subtilis, Japan]
GP2155 (''[[gene|nrnA]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
BKE29250 ([[gene|nrnA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATACTCCTTTATTG, downstream forward: _UP4_GAGTAGAGGGGAGACCCTCT
BKK29250 ([[gene|nrnA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATACTCCTTTATTG, downstream forward: _UP4_GAGTAGAGGGGAGACCCTCT
References
The protein
Protein family
NrnA oligoribonuclease family (single member, according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Cofactors]
Mn2+ [pubmed|28894100]
References
Reviews
References
Original publications