SubtiBank SubtiBank
Version comparison:

Mon May 16 2016 16:34:37 GMT+0200 (CEST)2025-05-24 10:59:14

locus

BSU06490

BSU_06490

outlinks

bsu

BSU06490

BSU_06490

[SW|Categories] containing this gene/protein

[SW|biosynthesis/ acquisition of nucleotides]

Gene

Coordinates on the chromosome (coding sequence)

705,441 -> 706,871

The protein

Catalyzed reaction/ biological activity

5-phospho-beta-D-ribosylamine diphosphate L-glutamate = L-glutamine 5-phospho-alpha-D-ribose 1-diphosphate H2O (according to Swiss-Prot)

5-phospho-β-D-ribosylamine + diphosphate + L-glutamate --> 5-phospho-α-D-ribose 1-diphosphate + H2O + L-glutamine (according to UniProt)

The protein

Additional information

:

subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]

subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Expression and Regulation

Operon

''[[protein|purE]]-[[protein|purK]]-[[protein|purB]]-[[protein|purC]]-[[protein|purS]]-[[protein|purQ]]-[[protein|purL]]-[[protein|purF]]-[[protein|purM]]-[[protein|purN]]-[[protein|purH]]-[[protein|purD]]'' [Pubmed|3036807]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|3036807]

Expression and Regulation

Regulation

repressed in the presence of purine nucleotides ([[protein|PurR]]) [Pubmed|7638212]

repressed in the presence of adenine or adenosine([[protein|PurR]]) [Pubmed|2536750]

repressed in the presence of guanine([[protein|G-box]]) [Pubmed|3036807]

Expression and Regulation

Regulatory mechanism

[PurR]]: transcription repression [Pubmed|7638212]

[[protein|PurR]]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750]

[[protein|G-box]]: transcription termination/ antitermination ([SW|riboswitch]) [http://www.ncbi.nlm.nih.gov/sites/entrez/3036807 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]

Expression and Regulation

Additional information

subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]

proteinLength

476

geneLength

1431

Gene

Coordinates

705,441 706,871

The protein

Protein family

[SW|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|Glutamine amidotransferase type-2 domain] (aa 12-231) (according to UniProt)

The protein

[SW|Cofactors]

Fe-S cluster [pubmed|29292548]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

BKE06490 ([[gene|purF]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCAAAAACGCCGCATTCTT, downstream forward: _UP4_TAAAACTTGAAAAATGACAT

BKK06490 ([[gene|purF]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCAAAAACGCCGCATTCTT, downstream forward: _UP4_TAAAACTTGAAAAATGACAT