2017-8-11 16:0:32025-05-22 13:55:23

locus

BSU14590

BSU_14590

geneLength

975

978

outlinks

bsu

BSU14590

BSU_14590

Gene

Coordinates

1,529,445 → 1,530,422

1,529,445 1,530,422

Gene

Phenotypes of a mutant

defects in sporulation and unable to grow on glucose as single carbon source [Pubmed|11976308]

poor growth [pubmed|28189581]

non-transformable [pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

Pyruvate [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine CO₂ (according to Swiss-Prot)

[dihydrolipoyllysine-residue acetyltransferase]-(R)-N⁶-lipoyl-L-lysine + H⁺ + pyruvate --> [dihydrolipoyllysine-residue acetyltransferase]-(R)-N⁶-(S⁸-acetyldihydrolipoyl)-L-lysine + CO₂ (according to UniProt)

The protein

Paralogous protein(s)

[[protein|AcoB]], [[protein|BkdAB]]

[[this]]

Biological materials

Mutant

GP459 (spc), available in [SW|Stülke] lab

GP459 (spc), available in [SW|Jörg Stülke]'s lab

BKE14590 ([[gene|pdhB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATTGTCATTTGCGCCA, downstream forward: _UP4_TAATCAAACTGCATAATCGA

BKK14590 ([[gene|pdhB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATTGTCATTTGCGCCA, downstream forward: _UP4_TAATCAAACTGCATAATCGA

Biological materials

lacZ fusion

pGP722 (in [[protein|pAC5]]), available in [SW|Stülke] lab

pGP722 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab

Biological materials

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] lab

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]

References

Original publications

9352926, 15378759, 12850135, 17218307, 18763711, 6414463, 11976308

9352926, 15378759, 12850135, 17218307, 18763711, 6414463, 11976308, 28189581

labs

[SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]