Mon May 16 2016 15:54:16 GMT+0200 (CEST)2025-05-11 15:27:50
locus
BSU06450
BSU_06450
product
phosphoribosylaminoimidazole
succinocarboxamide synthase
phosphoribosylaminoimidazole succinocarboxamide synthase
outlinks
bsu
BSU06450
BSU_06450
[SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of nucleotides], [SW|most abundant proteins]
Gene
Coordinates on the chromosome (coding sequence)
701,601 -> 702,326
The protein
Catalyzed reaction/ biological activity
ATP 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate L-aspartate = ADP phosphate (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (according to Swiss-Prot)
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate --> (2S)-2-[5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H+ + phosphate (according to UniProt)
Expression and Regulation
Operon
''[[protein|purE]]-[[protein|purK]]-[[protein|purB]]-[[protein|purC]]-[[protein|purS]]-[[protein|purQ]]-[[protein|purL]]-[[protein|purF]]-[[protein|purM]]-[[protein|purN]]-[[protein|purH]]-[[protein|purD]]'' [Pubmed|3036807]
Expression and Regulation
[SW|Sigma factor]
[[protein|SigA]] [Pubmed|3036807]
Expression and Regulation
Regulation
repressed in the presence of purine nucleotides ([[protein|PurR]]) [Pubmed|7638212]
repressed in the presence of adenine or adenosine([[protein|PurR]]) [Pubmed|2536750]
repressed in the presence of guanine([[protein|G-box]]) [Pubmed|3036807]
Expression and Regulation
Regulatory mechanism
[PurR]]: transcription repression [Pubmed|7638212]
[[protein|PurR]]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750]
[[protein|G-box]]: transcription termination/ antitermination ([SW|riboswitch]) [http://www.ncbi.nlm.nih.gov/sites/entrez/3036807 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]
Expression and Regulation
Additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
the mRNA is very stable (> 15 min) [pubmed|12884008]
proteinLength
241
geneLength
726
Gene
Coordinates
701,601 702,326
The protein
Paralogous protein(s)
[[this]]
The protein
additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Biological materials
Mutant
BKE06450 ([[gene|purC]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGGAAGGCCTCCTAACCC, downstream forward: _UP4_TTCAATAGACTGGGAGGCAT
BKK06450 ([[gene|purC]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGGAAGGCCTCCTAACCC, downstream forward: _UP4_TTCAATAGACTGGGAGGCAT